长期有氧运动增加自发性高血压大鼠肾脏内源性硫化氢的生成技术总结.doc

长期有氧运动增加自发性高血压大鼠肾脏内源性硫化氢的生成 王 兵1，侯 俊2，谷 崎1(1西安工业大学体育学院。陕西省西安市 710032；2洛阳师范学院体育学院，河南省洛阳市 471022) 引用本文：王兵，侯俊，谷崎. 长期有氧运动增加自发性高血压大鼠肾脏内源性硫化氢的生成[J].中国组织工程研究，2016，20(27): 4090-4097. DOI: 10.3969/j.issn.2095-4344.2016.27.020 ORCID: 0000-0003-1347-6596(王兵) 文章快速阅读： 文题释义： 自发性高血压大鼠：是用一只收缩压持续在150-175 mm Hg的雄性Wistar京都种大鼠与收缩压为130-140 mm Hg的雌性Wistar京都种大鼠进行交配，得到收缩压都 150 mm Hg的有效子代，再选用血压较高的子代大鼠进行近亲交配，依次进行这种选择性近亲交配20代以此来获得稳定的高血压遗传性，从而建立了自发性高血压大鼠品种，目前该选择手段和路径得到世界相关领域专家学者的高度认可和广泛使用。 硫化氢(H2S)：H2S是硫的氢化物中最简单的一种。肾脏中关于H2S生成的3个酶，分别是胱硫醚ββ合酶；3-巯基丙酮酸硫基转移酶；胱硫醚γ分解酶 主题词： 模型，动物；硫化氢；谷胱甘肽；组织工程 缩略语： 硫化氢：hydrogen sulfide，H2S Long-term aerobic exercise enhances the production of endogenous hydrogen sulfide in the kidney of spontaneously hypertensive rats Wang Bing1, Hou Jun2, Gu Qi1 (1School of Physical Education, Xi’an Technological University, Xi’an 710032, Shaanxi Province, China; 2School of Physical Education, Luoyang Normal University, Luoyang 471022, Henan Province, China) Abstract BACKGROUND: It is well known that long-term aerobic exercise alleviates renal dysfunction in spontaneously hypertensive rats. However, the underlying molecular mechanism remains unclear. OBJECTIVE: To investigate the effect of long-term aerobic exercise on endogenous formation of hydrogen sulfide in the kidney of spontaneously hypertensive rats. METHODS: Rat models of long-term aerobic exercise were established and randomly assigned to four groups: Wistar-Kyoto (WKY) rat static group, WKY rat exercise group, spontaneously hypertensive rat static group and spontaneously hypertensive rat exercise group. Moderate-intensity exercise on treadmill was given for 12 weeks. At 24 hours after model establishment, weight was weighted. Blood pressure was detected in the caudal artery. Blood and urine were collected for measuring biochemical indicators related to kidney functions. The degree of glomerular sclerosis was observed. Hydrogen sulfide production activity was detected in the kidn