捕获和释放活性细胞的方法试题.ppt

Capture and release live cells 汇报人：于倩茹 LIVE CELLS Circulating Tumors Cells (CTCs) T Lymphocytes Circulating Tumors Cells (CTCs) Circulating tumor cells (CTCs) are cancer cells that break away from either a primary tumor or a metastatic site and circulate in the peripheral blood as the cellular origin of metastasis. Notion: Why? Detecting and analyzing CTCs Provide insightful information in assessing the disease status CTCs provide convenient access to all disease sites 现代技术中捕获和释放CTCs的主要方法 根据分离原理 基于物理性质分离 基于亲和识别分离 基于亲和识别分离 利用癌细胞与正常血细胞的差别 密度 变形性 粘附性 尺寸 基于物理性质分离 核酸适配体 抗体 Nanostructure Embedded Microchips for Detection, Isolation, and Characterization of Circulating Tumor Cells 基于亲和识别-抗体-实例 Working mechsnism: When the two fabric strips of a Velcro fastener are pressed together, tangling between the hairy surfaces on two strips leads to enhanced binding. The proof-of-concept demonstration of the NanoVelcro cell-affinity assay lies in the use of a SiNS, which allows for Velcro-like interactions between the SiNS and nanoscale cell-surface components. Here, anti-EpCAM was grafted onto the SiNS as the capture agent, conferring specificity to such a cell-affinity assay for recognizing CTCs . Anti-EpCAM is grafted onto a SiNS to confer specificity for recognizing CTCs. The anti-EpCAM grafted SiNS was fabricated through three continuous steps: (i) introduction of densely packed silicon nanowires onto a silicon wafer (ii) silane treatment and covalent conjugation of streptavidin onto the SiNS (iii) grafting of biotinylated anti-EpCAM onto the streptavidin-coated SiNS. (a) An anti-EpCAM-coated SiNS was employed to achieve significantly enhanced capture of CTCs in contrast to (b) an anti-EpCAM-coated flat silicon substrate。 T Lymphocytes T lymphocytes have fundamental functions in immune responses to many diseases, such as pathogenic infections , malignancies , and autoimmune diseases. Detections of targeted T lymphocytes provide important i