人体细胞和肠道微生物数量要点.pptx

黄文龙 2016-06-16 1.Origin of prevalent claims in the literature on the number of bacterial cells In humans 2.Distribution of bacteria in different human organs 3.Concentration of bacteria in the colon 4.The number of human cells in a “standard” adult male A mass-based order-of-magnitude estimate for this number assumes a 10 2 kg man, which is divided by the mass of a “representative” mammalian, cell 10 -12 -10 -11 kg (assuming cell volumes of 1,000-10,000 μm 3 , spectively), thus arriving at 10 13 - 10 14 cells. One study used a DNA-centered method (Baserga, 1985). The method begins by estimating the number of cells in the body of a 25g mouse by dividing the total amount of DNA (stated to be 20 mg) by the DNA content of one diploid mouse cell(6·10 -12 g DNA per cell) to get ≈3·10 9 cells in a 25 g mouse. Then extrapolate from mouse to human by using the ratio of masses to get ≈10 13 cells in the human body. This method excludes cells that do not contain DNA, such as red blood cells and platelets. Summing over a total of 56 cell categories (Bianconi et al., 2013) resulted in an overall estimate of 3.7·10 13 humancells in the body (SD 0.8·10 13 , i.e. CV of 22%) 5.Updated inventory of human cells in the body We focus on the six cell types that were recently identified (Bianconi et al., 2013) to comprise 97% of the human cell count: red blood cell accounting for 70%), glial cells (8%), endothelial cells (7%), derma fibroblasts (5%), platelets (4%) and bone marrow cells (2%). The other 50 cell types account for the remaining 3%. 5.Updated inventory of human cells in the body Red blood cells: (Boer, 1984; Feldschuh and Enson, 1977; Nadler et al., 1962; Snyder et al., 1975) 4.9L (Ambayya et al., 2014; Dosoo et al.,2012; Nordin et al., 2004; Pekelharing et al., 2010; Volkmer and Heinemann, 2011;Wakeman et al., 2007) 5.0·10 12 cells/L (SEM 1.2%, CV 7%) (SEM 1.6%, CV 9%) This led to a total of 2.5·10 13 red blood cells (SEM 2%, CV 12%) This is similar to the ea