Livin、Beclin1在上皮性卵巢癌中的表达及与肿瘤侵袭转移的关系.docVIP

Livin、Beclin1在上皮性卵巢癌中的表达及与肿瘤侵袭转移的关系 　　[摘要] 目的 探讨Livin、Beclin1在上皮性卵巢癌中的表达及与肿瘤侵袭转移的关系。方法 选取于我院及吉林大学附属医院诊治的65例卵巢上皮性肿瘤患者和正常卵巢组织标本蜡块15例。将切除的卵巢组织以免疫组化法检测Livin及Beclin1蛋白表达情况，分析与卵巢癌组织分化、淋巴结转移及临床分期的关系。结果 Livin在正常卵巢组织几乎不表达（6.7%）、在卵巢癌组织中表达最高（68.6%）；Beclin1在正常卵巢组织表达最高（93.3%），在卵巢癌组织中表达最低（37.1%）。卵巢癌组织分化、淋巴结转移及临床分期与Livin与Beclin1表达程度相关（P 0.05）。 结论 Livin、Beclin1在上皮性卵巢癌中的表达与肿瘤侵袭转移中起重要作用，可成为卵巢癌治疗的新靶点。 　　[关键词] 上皮性卵巢癌；Livin；Beclin1；免疫组化 　　[中图分类号] R737.31 [文献标识码] B [文章编号] 1673-9701（2014）23-0135-03 　　[Abstract] Objective To explore the expression of Livin and Beclin1 in epithelial ovarian cancer and their relations with tumor invasion and metastasis. Methods A total of 65 patients with epithelial ovarian cancer who were admitted and treated in our hospital and Jilin University Affiliated Hospital， plus 15 blocks of normal ovarian tissue samples were selected. Excised ovarian tissues were tested via immunohistochemistry to test the protein expression of Livin and Beclin1， and their relations with tissue differentiation of ovarian cancer， lymphatic metastasis and clinical staging were analyzed. Results Positive rate of expression of Livin， rare expression in normal ovarian tissues （6.7%）， and the highest expression rate in tissues of ovarian cancer （68.6%）； Positive rate of expression of Beclin1， the highest expression rate in normal ovarian tissues （93.3%） and the lowest in tissues of ovarian cancer （37.1%）. Tissue differentiation of ovarian cancer， lymphatic metastasis and clinical staging were interrelated with the expression degree of Livin and Beclin1 （P0.05）. Conclusion Expression of Livin and Beclin1 in epithelial ovarian cancer and tumor invasion and metastasis plays an important role and may become a new target in the treatment of ovarian cancer. 　　[Key words] Epithelial ovarian cancer； Livin； Beclin1； Immunohistochemistry 　　卵巢癌作为女性生殖系统三大恶性肿瘤之一，死亡率最高，5年生存率不足30%。在肿瘤的发生、发展中细胞凋亡受阻是主要机制，可使化疗效果大打折扣。Livin基因属于凋亡抑制蛋白家族，可抑制细胞凋亡，与多种肿瘤的恶性程度与预后有关[1]。Beclin1基因可控制自噬活动，抑制肿瘤的生长，其机制为提高细胞自