养阴活血方药对糖尿病大鼠肝脏的氧化应激水平及病理改变的影响.docVIP

养阴活血方药对糖尿病大鼠肝脏的氧化应激水平及病理改变的影响 　　[摘要] 目的 探讨养阴活血方药对糖尿病大鼠肝脏氧化应激水平及病理改变的影响。 方法 采用腹腔注射链脲佐菌素（STZ）造糖尿病大鼠模型，挑选造模成功的大鼠，随机分为四组：糖尿病模型组；中药6周组，中药8周组，中药10周组，另外随机选出8只作为正常对照组，造模成功后分别于6、8、10周取材，测定肝组织氧化指标并进行病理观察。 结果 与正常对照组比较，糖尿病模型组的丙二醛（MDA）的含量明显增加（P 0.05）。病理学观察，模型组肝细胞索及肝小叶结构紊乱，炎症细胞增多，说明肝组织有损伤。与模型组比较，中药干预组各阶段形态学改变均有改善。 结论 养阴活血方药具有一定的抗氧化能力，抑制氧化应激，对糖尿病大鼠肝脏具有一定的保护作用。 　　[关键词] 糖尿病；大鼠；肝脏；氧化应激；养阴活血方药 　　[中图分类号] R587.2 [文献标识码] A [文章编号] 1673-7210（2014）08（b）-0039-04 　　Effects of Nourishing Yin and Promoting Blood Flow Recipe on oxidative stress level and pathologic changes in diabetic rat liver 　　YANG Xiaohui1 LI Shuzhen2 SONG Cuirong3 CUI Maoxiang2 LIANG Jinhuan2 　　1.Scientific Research Department， Cangzhou Medical College， Hebei Province， Cangzhou 061001， China； 2.Foundation Department， Cangzhou Medical College， Hebei Province， Cangzhou 061001， China； 3.Cavitas Oris Department， Cangzhou Medical College， Hebei Province， Cangzhou 061001， China 　　[Abstract] Objective To discuss the effects of Nourishing Yin and Promoting Blood Flow Recipe on oxidative stress level and pathologic changes in diabetic rat liver. Methods Diabetic rats were induced by intraperitoneal streptozotocin （STZ） injection. Successfully-established model rats were selected and randomly divided into four groups： diabetic group， traditional Chinese medicine （TCM） six-week intervention group， TCM eight-week intervention group， TCM ten-week intervention group. In addition， eight normal rats were randomly chosen as normal control group. Rat livers were extracted respectively after six weeks， eight weeks and ten weeks to determine oxidation index and undertake pathologic observation. Results Compared with normal control group， contents of malonaldehyde （MDA） in rat livers of diabetic group rose significantly （P 0.05）. By pathology observation， liver cell cords and hepatic lobules structure of diabetic group were chaotic， with inflammatory cells increasing， which indicated that there were injuries in hepatic tissue. Comp