龙辐麦3号小麦品种hmw-gsnull和1近等基因系间品质差异的研究.docVIP

龙辐麦3号小麦品种HMW-GS Null和1近等基因系间 品质差异的研究 张莉丽1，张延滨2，宋庆杰2，赵海滨2，于海洋2，张春利2，辛文利2，肖志敏2 （1哈尔滨师范大学生命与环境科学学院，哈尔滨150025；2黑龙江省农业科学院作物育种研究所，哈尔滨150086） 摘要：【目的】确定Glu-A1位点高分子量麦谷蛋白亚基（HMW-GS） Null和1间的遗传差异。【方法】利用生化标记和选择性回交的方法将1亚基转移到了黑龙江省小麦品种龙辐麦3号中，获得了龙辐麦3号的N亚基和1亚基的HMW-GS近等基因系。2004和2005年这2个近等基因系被种植在黑龙江省农科院育种所的实验地里，田间设计采用双列对比排列，4次重复。【结果】两年的品质分析结果表明，1亚基龙辐麦3号与N亚基龙辐麦3号两年平均数相比，其面粉蛋白质含量、干面筋和吸水率在统计学上无显著差异；面筋指数、沉降值、沉降值/干面筋、形成时间、稳定时间和断裂时间分别提高5.8%、9.3%、8.6%、127.3%、79.2%、53.6%，而湿面筋/干面筋和软化度分别低 1.7%和16.5%。【结论】在5＋10亚基的遗传背景下，1亚基对面筋强度仍有较大的影响，因此在选育强筋小麦时，1亚基也是必需被考虑的亚基之一。 关键词：小麦；品质；麦谷蛋白；近等基因系 Study on the Quality of NILs of Wheat Cultivar Longfumai 3 with HMW-GS Null and 1 Subunits ZHANG Li-li1, ZHANG Yan-bin2, SONG Qing-jie2, ZHAO Hai-bin2, YU Hai-yang2, ZHANG Chun-li2, XIN Wen-li2, XIAO Zhi-min2 (1 College of Life and Environment Science, Harbin Normal University, Harbin 150025; 2 Crop Breeding Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086) Abstract:【Objective】 T determine the genetic differences in high molecular weight glutenin subunits (HMW-GS) Null and 1. 【Method】HMW-GS 1 was introduced into Longfumai 3 by consecutive backcrosses with biochemical marker assisted selection. The near isogenic lines (NILs) of HMW-GS Null and 1 were obtained and grown the of Crop Breeding Institute of Heilongjiang Academy of Agricultural Science experimental fields in 2004 and 2005. The field experiments were designed using a two line-contrast arrangement with four replicates. 【Result】 The twoyear average results showed that the statistical differences in quality parameters between Longfumai 3 with 1 subunit and with Null were not significant in flour protein content, dry gluten content, water absorption. However the gluten index, Zeleny sedimentation, the ratio of sedimentation/dry gluten, development time, stability and breakdown time in the NIL with 1 subunit were increased by 5.8%, 9.3%, 8.6%, 127.3%, 79.2%,