厦门市副溶血性弧菌的脉冲场电泳分析及数据库建立.docVIP

厦门市副溶血性弧菌的脉冲场电泳分析及数据库建立 　　[摘要] 目的 建立厦门市副溶血性弧菌分离株的脉冲场凝胶电泳（PFGE）数据库，分析不同来源菌株的同源性关系。 方法 203株菌株的基因组DNA经SfiⅠ酶切、脉冲场电泳后，利用Bionumerics软件分析电泳图谱。 结果 203株菌株可分成149种不同的PFGE型，91.13%（185株）的菌株具有独特的带型，同时也有少数带型包含的菌株数较多。对于日常监测中分离得到的菌株，PFGE分型结果与水产品的种类、采样时间基本无相关性，79个菌株可分成74个PFGE带型；从历年食物中毒应急监测中分离得到的菌株，其PFGE型相对较少，124株中只有76个PFGE型。应用Bionumerics计算D值为99.81%。 结论 建立了厦门市副溶血性弧菌的PFGE数据库，为本市副溶血性弧菌的监测和疫情的应急处理提供了科学依据。 　　[关键词] 副溶血性弧菌；脉冲场凝胶电泳；分子分型 　　[中图分类号] R378.3 [文献标识码] A [文章编号] 1674-4721（2014）08（a）-0007-04 　　Molecular analysis and database establishment of Vibrio parahaemolyticus by pulsed field gel electrophoresis in Xiamen 　　WENG Qin-yun YOU Xiao-wei ZHANG Jian-mei HUANG Jian-wei 　　Center for Disease Control and Prevention of Xiamen City，Xiamen 361021，China 　　[Abstract] Objective To establish pulsed field gel electrophoresis（PFGE）database of Vibrio parahaemolyticus and to analyse the homology during the strains from different sources. Methods The DNA samples of 203 Vibrio parahaemolyticus strains were digested with SfiⅠand then were analyzed by PFGE and bionumerics software. Results 203 strains were divided into 149 distinctive PFGE patterns 91.13% （185 strains） of strains owned unique PFGE pattern，and a few of strains owned more strains.79 strains from surveillance were divided into 74 PFGE patterns，which indicated that there was no relationship between the typing results and aquatic product，sampling time.124 strains from food poisoning were divided into 76 PFGE patterns.The D value was 99.81% calculated by bionumerics software. Conclusion The PFGE database of Vibrio parahaemolyticus in Xiamen is established，which providing scientific basis for surveillance and emergency response of Vibrio parahaemolyticus in the city. 　　[Key words] Vibrio parahaemolyticus；Pulsed field gel electrophoresis；Molecular typing 　　副溶血性弧菌是一种嗜盐性革兰氏阴性弧菌，广泛存在于海水和海产品中，进食含有该菌的食物，可引起严重的胃肠炎反应[1]，目前是我国沿海地区常见的食物中毒病原菌。脉冲场凝胶电泳（pulsed field gel electrophoresis，PFGE）技术是一种非常重要且应用广泛的分子分型技术，可用于评价副溶血性弧菌菌株之间的遗传多样性及来源于环境的菌株与分离