微柱凝胶技术检测血型不规则抗体的临床应用.docVIP

微柱凝胶技术检测血型不规则抗体的临床应用 　　[摘要] 目的 分析微柱凝胶技术对输血患者进行血型不规则抗体的筛查情况及临床应用。 方法 对2010年1月~2013年12月于桂林医学院附属医院输血患者29 208例进行不规则抗体检测，统计分析阳性结果，鉴定阳性抗体的特异性。 结果 29 208例患者中，检出不规则抗体阳性者90例，阳性率为0.31%。特异性抗体以Rh血型系统居多，抗-D抗体占2.22%、抗-E抗体占21.11%、抗-Ec抗体占11.11%；MNSs系统抗-Mur抗体11例（12.22%）、抗-M抗体7例（7.78%）；Lewis系统抗-Lea抗体占8.89%；冷抗体占7.78%。非特异性抗体占13.33%。 结论 输血前对患者血液进行血型不规则抗体筛查并鉴定其特异性，输注不含相应抗原的血液，避免溶血性输血反应的发生，对确保输血安全有效有重要的临床意义。 　　[关键词] 微柱凝胶技术；不规则抗体筛查；安全输血；输血反应 　　[中图分类号] R457.1[文献标识码] A[文章编号] 1673-7210（2014）06（a）-0091-03 　　 　　The clinical application of irregular antibody screening by microtube gel technology 　　NING Fang SU Mingli DENG Meiying▲ 　　Department of Transfusion, Affiliated Hospital of Guilin Medical University, Guangxi Zhuang Autonomous Region, Guilin 541001, China 　　[Abstract] Objective To analyze the situation and clinical application of microtube gel technology (MGT) in the irregular antibody screening of patients before blood transfusion. Methods 29 208 patients from January 2010 to December 2013 in Affiliated Hospital of Guilin Medical University were screened for irregular antibody. The results were statistical analyzed to identify the specificity of positive antibody. Results Among 29 208 patients, there were 90 cases with irregular antibody, the positive rate was 0.31%. Rh blood type system accounted for the most part of the specific antibodies: 2.22% for anti-D antibody, 21.11% of anti-E antibody and 11.11% of anti-Ec antibody. 11 cases of Anti-Mur of MNSs system accounted for 12.22% of the total and 7 cases of anti-M antibody accounted for 7.78%. 8.89% was for Anti-Lea antibody in Lewis system and 7.78% for cold antibody. And the non-specific antibodies accounted for 13.33%. Conclusion Irregular antibody should be screened and its specificity should also be identified before transfusion. To transfuse the blood without the corresponding antibodies can prevent transfusional hemolytic reaction, which is of great significance in safe and effective transfusion in clinical work.