正交试验优选温阳通便颗粒中挥发油提取工艺.docVIP

正交试验优选温阳通便颗粒中挥发油提取工艺 　　摘要：目的 优选温阳通便颗粒中当归、肉桂挥发油的最佳提取工艺条件。方法 采用水蒸气蒸馏轻油装置加乙醚萃取的方法提取当归、肉桂挥发油，以挥发油收率作为评价指标，选择浸泡时间、加水量及提取时间为主要影响因素，应用L9（34）正交试验设计，选取最佳提取工艺条件。结果 水蒸气蒸馏法提取当归、肉桂挥发油的最佳工艺条件为：药材浸泡3 h，加10倍量水，蒸馏提取6 h。结论 本试验优选所得提取工艺条件稳定可行。 　　关键词：当归；肉桂；挥发油；正交试验 　　DOI：10.3969/j.issn.1005-5304.2014.08.022 　　中图分类号：R283.5 文献标识码：A 文章编号：1005-5304（2014）08-0076-03 　　Optimization of Extraction Process of Volatile Oil from Wenyang Purgation Granules by Orthogonal Design DU Qiu， TAN Xuan-zhong， DING Shu-qing， XIA Qin， ZHAO Ying （The Third Affiliated Hospital of Nanjing University of TCM， Nanjing 210001， China） 　　Abstract：Objective To optimize the extraction conditions of volatile oil from Angelica Sinensis and Cassia Bark in Wenyang purgation granules. Methods The volatile oil was extracted from Angelica Sinensis and Cassia Bark by the method of steam distillation light oil device with diethyl ether extraction. The yield of the volatile oil was chosen as the evaluation index. The time of dip in water， the ratio of water to herbal medicine material and the time of distillation were used as the main factors. The optimum extraction conditions were investigated by the L9（34） orthogonal design. Results The optimal conditions for extraction process of volatile oil from Angelica Sinensis and Cassia Bark in Wenyang purgation granules were as follows：the time of dip in water was 3 h；the ratio of water to herbal medicine material was 10∶1；the time of distillation was 6 h. Conclusion The optimized conditions of extraction process are stable and feasible. 　　Key words：Angelica Sinensis；Cassia Bark；volatile oil；orthogonal experiment 　　温阳通便颗粒是本院肛肠科经验方，由肉苁蓉、淫羊藿、当归、厚朴、肉桂、半夏等组成，具有温通肾阳、养血润燥、润肠通便等功效，用于治疗长期滥用泻药，苦寒日久，五脏阳气受损，肠失温煦所致的脾肾阳虚型便秘。方中当归甘温，具有补血调血、调经止痛、润肠通便功效，配伍肉苁蓉，养血润燥、增水行舟，增强通便疗效；肉桂具有引火归源、补火助阳、活血通经、散寒止痛功效，助肉苁蓉、当归温补下元。挥发油是当归、肉桂的主要有效成分[1-2]，其挥发性较强，在提取及制剂工艺过程中易损失，为保证复方药效，需将此类成分单独提取处理后入药。本研究采用水蒸气蒸馏法提取当归、肉桂混合挥发油，并用正交试验优选提取工艺条件，使有效成分最大程度地提取出来，从而保证该复方制剂药效的发挥。 　　基金项目：南京市中医院科研项目（YJ201408） 　　通讯作者：谈?忠，E-m