《陈新-生理科学实验教学资料》离子与药物对离体蟾蜍心脏活动的影响.docVIP

离子与药物对离体蟾蜍心脏活动的影响 冯磊 杨雨喆 吴笑 浙江大学医学院2003级基础医学0301班1组 3031851012 [摘要] 目的 学习灌流蟾蜍离体心脏方法，观察高钾、高钙、低钙、肾上腺素、乙酰胆碱等因素对心脏活动的的影响。方法 制备蟾蜍离体心脏标本, 分别灌流CaCl2、KCl、肾上腺素+普奈洛尔及乙酰胆碱, 用张力换能器与PCLab生物信号采集处理系统描记心搏曲线并记录心搏变化。 结果 将插管内的任氏液全部更换为无钙任氏液后，心脏的舒张期张力无明显改变，收缩期张力减小，心率增加；滴加30g/L CaCl2溶液1～2滴后，收缩期、舒张期张力增加，心率显著减小（p0.05）；在任氏液中滴加10g/L KCl溶液1～2滴后，心脏的收缩期张力显著减小（p0.05），舒张期张力增加，心率非常显著减小（p0.01）；在任氏液中滴加0.1g/L 肾上腺素溶液1～2滴后，心脏的收缩期张力增加，舒张期张力、心率基本不变；在任氏液中滴加0.1g/L 肾上腺素溶液1～2滴后，心脏的舒张期张力基本不变，收缩期张力显著增加，心率轻度增加；待心搏曲线稳定后，向灌流液中加10g/L 普奈洛尔溶液1～2滴，可见心脏的舒张期张力基本不变，收缩期张力略有减小，心率基本不变。待心搏曲线稳定后，再向灌流液中加0.1g/L 肾上腺素溶液1～2滴，心搏曲线无明显变化；在任氏液中加10-2g/L 乙酰胆碱溶液1～2滴后，心脏的舒张期张力增加，收缩期张力显著减小（p0.05），心率则有非常显著减小（p0.01）。 结论 心肌细胞外高Ca2+时心肌收缩性增强；高钾使心肌收缩性减弱；肾上腺素使心输出量增加, 心肌收缩性增强，而普奈洛尔可不可逆转性的抑制肾上腺素的作用；乙酰胆碱可使心肌收缩性降低。 [关键字] 蟾蜍 离体心脏灌流肾上腺素 乙酰胆碱 [Abstract] Objective study fills flows the toad to the body heart method, observes K, factor and so on Ca, low calcium, adrenalin, acetylcholin to the cardiac activity influence. Methods preparation toad leaves the body heart specimen, Fills separately flows CaCl2, KCl, the adrenalin + propranolol and the acetylcholin, Wrestles the curve and the record heart with the tensity transducer and the PCLab biology signal gathering processing system description heart wrestles the change. Results it will insert a tube no matter what the fluid replaces completely after does not have the calcium no matter what the fluid, the heart diastole time tensity does not have the obvious change, the contraction time tensity reduces, heart rate increase; Adds by drops 30g/After L the CaCl2 solution 1 ~ 2 drops, the contraction time, the diastole time tensity increases, the heart rate remarkably reduces (p0.05); In no matter what the fluid adds by drops 10g/After L the KCl solution 1 ~ 2 drops, the heart contraction time tensity remarkably reduces (p0.05), the diastole time tensity increases, the heart rate extremely remarkably reduces (p0.01); In no matter what the fluid adds by drops 0.1g/After the L adrenalin solution 1 ~ 2