农药吡虫啉对牛蛙外周血红细胞的影响.docVIP

农药吡虫啉对牛蛙外周血红细胞的影响 生命与环境科学学院 潘海乙，南旭阳*，陈院豪，陈坤 摘 要：本研究以不同浓度的农药吡虫啉溶液为染毒药物，以牛蛙为实验对象，研究了牛蛙在0.005g/L到 0.04 g/L吡虫啉溶液的胁迫下，牛蛙外周血中血红细胞数、红细胞微核率、核异常率以及总核异常率的变化。结果表明：吡虫啉对牛蛙血红细胞数、红细胞微核率、核异常率以及总核异常率均有一定的影响。吡虫啉浓度为0.04g/L时，染毒组的血红细胞数不仅低于对照组，而且达到了差异显著水平（p(0.05）或差异极显著水平（p(0.01）。吡虫啉浓度≥0.02g/L时，所有染毒组的血红细胞微核率不仅高于对照组，而且均达到了差异显著水平（p(0.05）或差异极显著水平（p(0.01或 p(0.001）。当吡虫啉浓度≥0.01g/L时，所有染毒组的血红细胞核异常率及总核异常率均高于对照组，而且均达到了差异显著水平（p(0.05）或差异极显著水平（p(0.01或 p(0.001）。 关键词：牛蛙；吡虫啉；血红细胞；微核；核异常 Effect of pesticide（Imidacloprid） on the Erythrocyte of Bullfrog（Rana catesbeiana） PAN Hai-yi，NAN Xu-yang，CHEN Yuan-hao，CHEN Kun (School of Life and Environment Science, Wenzhou University, Wenzhou 325035, China) Abstract: In this study, the pesticide (imidacloprid) solution was taken as the target poison and bullfrog(Rana catesbeiana) was taken as the experimental animal. The imidacloprid concentrations were set in the range from 0.005g/L to 0.04 g/L. The effects of imidacloprid on the numbers 、micronuclei rates、nuclear anomalies rates and total nuclear anomalies rates of erythrocyte in bullfrog were studied.The results showed that the imidacloprid could affect the numbers 、micronuclei rates、nuclear anomalies rates and total nuclear anomalies rates of erythrocyte in bullfrog. When the imidacloprid was 0.04g/L, the numbers of erythrocyte in the experimental groups(treated with imidacloprid) were lower than the number of erythrocyte in the contrast group. And there were significant difference between the experimental groups and contrast group(p(0.05 or p(0.01) . When the imidacloprid were higher（(0.02g/L）, the micronuclei rates of erythrocyte in the experimental groups were higher than in the contrast group. And the difference between the experimental groups and contrast group wre significant (p(0.05，p(0.01 or p(0.001) . When the imidacloprid were higher（(0.01g/L）, the nuclear anomalies rates and total nuclear anomalies rates of erythrocyte in the experimental groups were higher than in the contrast group