杨梅素减轻高糖诱导的血管内皮细胞氧化应激损伤-第三军医大学学报.doc

杨梅素减轻高糖诱导的血管内皮细胞氧化应激损伤 梁馨予，张 婷，周 永，施琳颖，陈军丽，王 丽，康 超，朱俊东，糜漫天 （400038重庆，第三军医大学军事预防医学院营养与食品安全研究中心，重庆市营养与食品安全重点实验室，重庆市医学营养研究中心） [摘要] 目的 探讨杨梅素对高糖诱导的血管内皮细胞氧化应激损伤的影响。方法 体外培养原代人脐静脉内皮细胞uman Umbilical Vein Endothelial Cells，HUVECs)，分为正常对照组（葡萄糖浓度5.56mmol/L）、甘露醇高渗对照组（5.56 mmol/L葡萄糖+27.8 mmol/L甘露醇）、高糖处理组（葡萄糖浓度33.36mmol/L）、杨梅素保护组（0.1～10μmo/L杨梅素+33.36mmol/L葡萄糖），处理48 h后，CCK-8比色法检测细胞活力，Annexin-FITC/PI双染流式细胞术过氧化氢））） R151.2; R543.3; R364.5 【文献标识码】 A Myricetin attenuates high glucose-induced oxidative stress in vascular endothelial cells Liang Xinyu, Zhang Ting, Zhou Yong, Shi Linying, Chen Junli, Wang Li, Kang Chao, Zhu Jundong, Mi Mantian (Research Center for Nutrition and Food Safety, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, Third Military Medical University, Chongqing, 400038, China) 【Abstract】 Objective To the effects of myricetin on oxidative stress in vascular endothelial cells induced by high glucose. Methods Human umbilical vascular endothelial cells (HUVECs) were treated by 5.56 mmol/L glucose as normal control, 5.56 mmol/L glucose plus 27.8 mmol/L mannitol as an osmotic control, or 33.36 mmol/L glucose for 48 h in the absence and presence of myricetin (0.1 to 10 μmol/L). Cell viability and apoptosis were detected by CCK-8 assays and annexin V-FITC/PI double staining using flow cytometry, respectively. In addition, the intracellular reactive oxygen species (ROS), malondialdehyde (MDA) content and the activity of superoxide dismutase (SOD) and catalase (CAT) in the culture supernatants were measured. Results Compared with the normal control group, high glucose significantly decreased cell viability and the activity of SOD and CAT (P0.05), while increased the apoptotic rate, intracellular ROS and MDA content in HUVECs (P0.05). However, myricetin pretreatment significantly inhibited these high glucose-induced above phenomena (P0.05). Conclusion Myricetin attenuates high glucose-induced oxidative stress and apopt