迷你文心兰组培快繁技术研究.docVIP

迷你文心兰组培快繁技术研究 　　摘要 以迷你文心兰侧芽为材料，系统研究了外植体取材与消毒，诱导、增殖、分化、生根培养基筛选等组培快繁技术，旨在为迷你文心兰种苗生产奠定技术基础。结果表明：采集当年新生侧芽，长4～6 cm，采用0.1% HgCl2溶液二次消毒的方法，有利于降低外植体污染率，提高成活率。类原球茎诱导以MS+6-BA 4.0 mg/L+NAA 1.0 mg/L+蔗糖20 g/L效果最好，培养45 d最高诱导率达130%；增殖采用MS′+6-BA 1.0 mg/L+NAA 0.2 mg/L+蔗糖10 g/L附加5%苹果汁，培养60 d最高增殖倍数达40.7倍；分化采用MS′+6-BA 0.8 mg/L+NAA 0.1 mg/L+蔗糖20 g/L，培养60 d，56.7%类原球茎可分化出芽；试管苗生根以MS′+NAA 0.8 mg/L+蔗糖20 g/L附加5%香蕉泥效果最好，培养60 d，平均生根数3.4条，平均根长3.16 cm。 　　关键词 迷你文心兰；组培快繁；类原球茎 　　中图分类号 S682.3 文献标识码 A 文章编号 1007-5739（2016）03-0181-03 　　Abstract Taking Mini Oncidium bud as material，the explant and disinfection induced，proliferation，differentiation，rooting culture medium screening technology of tissue culture and rapid propagation were systematic studied，to laid the technical foundation of mini Oncidium seedling production. The results showed that acquisition when newborn buds，ca. 4～6 cm，with 0.1% HgCl2 solution secondary disinfection method，could reduce the rate of explants pollution，improve the survival rate. Effect of protocorm like body induction to MS+6-BA 4.0 mg/L+NAA 1.0 mg/L+sucrose 20 g/L was the best，culture 45 days with the highest induction rate of 130%；proliferation by MS′+6-BA 1.0 mg/L+NAA 0.2 mg/L+sucrose 10 g/L added with 5% apple juice，culture 60 d the highest proliferation multiple was 40.7 times；differentiation using MS′+6-BA 0.8 mg/L+NAA 0.1 mg/L+sucrose 20 g/L，cultivated for 60 d，56.7% of protocorm like bodies could bud differentiation；effect of rooting in MS′+NAA 0.8 mg/L+sucrose 20 g/L added with 5% banana mud was best，medium 60 days，average root number was 3.4 and the average root length was 3.16 cm. 　　Key words mini Oncidium；micropropagation；protocorm-like body 　　迷你文心兰以其小型、带有清香特点，成为办公桌乃至家庭摆设的一种时尚。在多年引种、杂交育种基础上，不断筛选和培育出适宜华南地区栽培的品种[1]，并建立了一套标准栽培技术[2]。通过组织培养方法批量提供品质优良且生长状态一致的种苗，是目前观赏兰科植物通用的种苗生产方式，是迷你文心兰产业化生产的基础。尽管对文心兰组培快繁相关报道较多[3-4]，但对迷你文心兰组培快繁研究相关报道较少，也未能建立较为完善的体系。试验在前期的研究基础上[5]，通过对迷你文心兰组培快繁相关技术的系统研究，建立了迷你文心兰离体快繁的技术体系，为迷你文心兰种苗生产奠定了技术基础。 　　1 材料与方法 　　1.1 试验材料 　　试验以玉香、金香为材