上皮间质转化在胃癌SGC7901细胞向胃癌干细胞转化过程中的作用.docVIP

上皮间质转化在胃癌SGC7901细胞向胃癌干细胞转化过程中的作用 邢会军1，赵艳军2，侯 雷1，孙 勇1，刘 朋1，李春辉3(承德医学院附属医院，1胃肠外科，2急诊科，3病理科，河北省承德市 067000) 引用本文：邢会军，赵艳军，侯雷，孙勇，刘朋，李春辉. 上皮间质转化在胃癌SGC7901细胞向胃癌干细胞转化过程中的作用[J].中国组织工程研究，2016，20(10):1426-1432. DOI: 10.3969/j.issn.2095-4344.2016.10.008 ORCID: 0000-0001-5257-766X(邢会军) 文章快速阅读： 文题释义： 上皮间质转化：是指上皮细胞失去其形态和功能后，逐渐转化为间质样细胞的过程，与肿瘤的侵袭、转移、复发以及耐药性密切相关。上皮间质转化的调控因素有很多，如E钙黏蛋白、转化生长因子β、Wnt信号通路、微RNA及转录因子等。 上皮间质转化过程抑制致癌基因引起的提前衰老。在上皮间质转化过程中的肿瘤可以拮抗化疗和免疫疗法，并且逃脱免疫系统的监视。最近的研究中发现上皮间质转化中的细胞可以获得类似于干细胞的特性，这些尚未分化的间质细胞从表达E变为表达N，并且表达Snail，蛋白酶，以及一些细胞全能性转录因子如Oct-4和Nanog。 Role of epithelial-mesenchymal transition in transformation of gastric cancer cells SGC7901 to gastric cancer stem cells Xing Hui-jun1, Zhao Yan-jun2, Hou Lei1, Sun Yong1, Liu Peng1, Li Chun-hui3 (1Department of Gastrointestinal Surgery, 2Department of Emergency, 3Department of Pathology, Affiliated Hospital of Chengde Medical University, Chengde 067000, Hebei Province, China) Abstract BACKGROUND: Studies have found that epithelial-mesenchymal transition is closely related with tumor invasion, metastasis, and drug resistance, but studies on the role of epithelial-mesenchymal transition in the transformation process of gastric cancer cells SGC7901 to gastric cancer stem-like cells are rarely reported. OBJECTIVE: To explore the effect of epithelial-mesenchymal transition in the transformation process of gastric cancer cells SGC7901 to gastric cancer stem-like cells. METHODS: Vincristine-induced SGC7901 cells were cultured and screened to prepare gastric cancer stem-like cells. CD44 phenotype, morphological changes, stem cell-related markers, and epithelial-mesenchymal transition related molecules were detected. RESULTS AND CONCLUSION: After passage, vincristine-induced SGC7901 cells presented with morphological changes, and clonal cell spheres generated after serum-free suspension culture. Meanwhile, the proportion of SGC7901 cells positive for CD44 was decreased. Expression levels of SOX2, OC