作物遗传育种书籍.pptVIP

利用4 个标记的引物扩增带型构成19 个品种的特异性指纹, 根据指纹图谱的差异使它们相互区分。 ③本实验室利用 20 对 SSR引物对75个河北省 不同植物类型花生地方品种遗传多样性进行分析。共检测到65个等位基因，品种间不同位点等位基因数目不等，范围为 2～6 个，平均 3.25个，其中以 PM15、PM377 的等位变异数最多，为 6个。通过计算20对引物在不同品种间的多态信息指数和聚类分析将河北省地方品种区分、归类。 分子标记在花生育种上的贡献 In order to develop a genetic linkage map for tetraploid cultivated groundnut, a total of 1,145 simple sequence repeat (SSR)markers were screened on two genotypes, TAG 24 and ICGV 86031 that are parents of a recombinant inbred line mapping population. Method Result 144 (12.6%) polymorphic markers were identi?ed and these ampli?ed a total of 150 loci. A total of 135 SSR loci could be mapped into 22 linkage groups (LGs). Material and method Three recombinant inbred lines (RILs) populations were constructed from three crosses with one common female parental line Yueyou 13, a high yielding Spanish market type. The four parents were screened with 1044 primer pairs designed to amplify SSRs and 901 primer pairs produced clear PCR products. Result The composite linkage maps consist of 22 composite linkage groups (LG) with 175 SSR markers (including 47 SSRs on the published AA genome maps), representing the 20 chromosomes of A. hypogaea. The total composite map length is 885.4 cM, with an average marker density of 5.8 cM. Experiment Purpose The objective of this study was to develop a comparative integrated map from two cultivated ×cultivated recombinant inbred line (RIL) mapping populations and to apply in mapping Tomato spotted wilt virus (TSWV) resistance trait in peanut. Result A total of 4,576 simple sequence repeat (SSR) markers were used for screening polymorphisms. A total of 324 markers were anchored on this integrated map covering 1,352.1 cM with 21 linkage groups (LGs). 分子标记的应用前景 通过分子标记技术研究花生种质资源的 DNA 分子多态性,从分子水平上揭示花生种质资源的遗传变异,筛选出适合花生种质资源指纹图谱鉴定的分子标记技术, 找出不同花生种质资源间的分子标记,直接为花生品种改良和种质资源的鉴定、保存提供可靠的遗传标记和新技术新方法,为下一步研究各种遗传标记的遗传连锁关系、分子标记辅助育种、遗传图谱构建、数量基因座定位及基因克隆打基础,对花生染色体组结构和功能基因组研究等方面也有重要意义。 谢 谢 * 分子标记在花生