分子生物学0幻灯片.pptVIP

The Nobel Prize in Physiology or Medicine 2006 for their (Craig C. Mello and Andrew Z Fire) discovery of RNA interference– gene silencing by double-stranded RNA. This phenomenon was called by cosuppression and post-transcriptional gene silencing (PTGS) in plants, RNA interference (RNAi) in animals such as nematodes and fruit flies. How does RNAi work? RNA-induced silencing complex , RISC RNase III-like enzyme Dicer cleaves dsRNA to produce double-stranded fragments of 21–23 base pairs with a 2-nucleotide overhang at the 3 end. siRNA: short interfering RNA RNA interference occurs when a cell e