Histological Techniques ISU Public Homepage Server组织学技术大学公共主页服务器.pptVIP

* Slide Other Methods Northern blotting – analyzes mRNA expression in tissue In situ hybridization – Visualizing gene activity (mRNA) directly in fixed cells or tissues Western blotting – analyzes proteins expressed in tissue Immunohistochemistry (IHC) or Immunocytochemistry (ICC) – labels proteins in fixed cells or tissues Northern Blotting Purpose: Can tell us if a particular gene is being expressed in a specific cell or tissue type or if there are changes in levels of expression General Procedure: Isolate mRNA from cells Separate mRNA with gel electrophoresis Blot (transfer) the mRN