Microbiology Primer Department of Engineering 微生物学底漆工程系.pptVIP

Microbiology Primer YSU – Weapons of Mass Destruction Historical Biogenesis vs. Spontaneous Generation 19th Century – Golden Age Germ Theory Pasteur Koch Lister Classification Animalia Plantae Fungi Protista Monera Comparison Eucaryotic Nuclear Membrane Diploid DNA Mitosis Organelles Present Simple Cell Wall Procaryotic No Nuclear Membrane Haploid No Mitosis Organelles Absent Complex Cell Membr. Procaryotic Organism Bacteria 0.5-6 ?m (human hair is about 90 ?m) Simple Stapylococcus is 1.2 ?m Shape Coccus Bacillus Spirillum Spirochete Useful Bacteria Acetobacter – vinegar Lactobacilli – yogurt, cheese Actinomyces – antibiotics Others Industrial alcohols, acids, enzymes, rubber and plastics Pathogenic Bacteria Grp A Strep B. pertussis S. aureus H. influenza Kl. pneumoniae L. pneumophila M. pneumoniae Salmonella typhi Plague Cholera Typhus Anthrax Q-Fever Clostridium Tularemia Glanders Bacteria Group Patterns Cell Membrane Gram Positive Gram Negative Bacteria Endospores Significance Sporulation is important in Bacillus Clostridium species. Botulism disease B. anthracis Bacteria Reproduction Binary fission Daughter cells Population doubles each generation Phase (important in disease) Lag Exponential Stationary Death Similar Organisms Rickettsiae Gram negative Difficult to stain Grow in living cells Treatable with antibiotics Q-Fever Rocky Mt. Fever Viruses Viruses Viruses Submicroscopic 0.02 - 0.3 ?m Either DNA or RNA Glycoprotein envelope Obligate parasite Viral Reproduction - RNA Replicates in cytoplasm Capsid dissolves RNA acts as messenger Cell systems harnessed for replication Animal Cell Prions BSE – 1986, also classified as a transmissible spongiform encephalopathies. vCJD Other Organisms Amoeba Flagellates Protozoans (dinoflagellates) – paralytic shellfish poisoning Plasmodium (malaria) Nutritional Patterns Saprophytic Live on dead organic material Holozoic Ingest solid food Parasitic Nutrients at expense of host Obligate Parasite Requires host for speci