Solid Phase MicroextractionSPME固相微萃取SPME.pptVIP

Solid Phase Microextraction(SPME) Samantha Keene Tuesday, 30 June 2009 Who Discovered SPME? Solid Phase Microextraction was invented in 1990 by Dr. Janusz Pawliszyn and his colleagues from the University of Waterloo in Canada. He invented this technique to “address the need for a fast, solvent-free, and field compatible sample preparation method”, which faster and more efficient is the name of the game in industry. What is an SPME? It consists of coated fibers that are used to isolate and concentrate analytes into a range of coating materials. After extraction, the fibers are transferred to an analytical instrument for separation and quantification of the target analytes. This is accomplished with the help of a syringe-like handling device that protects your sample while transferring from your sample to the instrument. This syringe-like device also protects your fiber during storage. More on SPME SPME is also a microextraction technique that, when compared to the sample volume, contains a very small amount of extraction solvent. SPME allows for an equilibrium to be reached between the sample matrix and the extracting phase rather than an exhaustive removal of the analytes to the extracting phase occurring. The extracting phase is permanently attached to a rod that is made out of different materials, which makes this approach practical. The amount of analyte adsorbed by the fiber depends on the thickness of the coating and on the distribution constant of the analyte. Extraction time depends on the length of time required to obtain precise extractions for the analytes with the highest distribution constants. How does SPME work? First, you draw the fiber into the needle. The needle is then passed through the septum that seals the vial. You then depress the plunger to expose the fiber to your sample or headspace above the sample. Organic analytes are then adsorbed to the coating on the fiber. After adsorption equilibrium is attained, which can be anywhere