Use and Operation of Vacuum Lines UCLA Chemistry 使用真空线路运行UCLA化学.pptVIP

Safe Use and Operation of Vacuum Lines Outline Starting Up Shutting Down Cleaning Manifold Changing Pump Oil Dealing with Liquid O2 Starting Up PPE Needed: Lab Coat, Gloves, Safety Goggles/Glasses 1. Ensure that the manifold stopcocks, as well as all seals, are properly greased. 2. Check to see that the vacuum trap is dry and clean. 3. Make sure that there is enough Liquid N2 on hand to fill the dewar. 4. Insert traps into empty dewars. 5. Ensure that all valves are closed, and start or open line to the vacuum pump. 6. Fill the dewar with liquid nitrogen. Wrap the top with a towel, cotton, glass wool, or aluminum foil. 7. Watch the gauge to ensure that the pressure is dropping. Notes for Starting Up Depending on whether you have one or two traps, you will have to refill the dewar(s) every 6-8 or 12 hours, respectively. Make sure the system is under vacuum while any portion is under liquid nitrogen. Otherwise, oxygen could condense, which could result in a serious explosion (see below). Shutting Down PPE Needed: Lab Coat, Gloves, Safety Goggles/Glasses 1. Remove the Liquid N2 dewar. 2. Shut off vacuum pump and vent the manifold. 3. Once the dewars are taken down, allow the system to thaw to room temperature before safely disposing of any solvents. Notes for Shutting Down Never vent the system to air or turn off vacuum when any portion of the system is still at liquid nitrogen temperature. This could condense oxygen which could result in a serious explosion (see below). Always make sure the liquid nitrogen is removed prior to turning off or venting vacuum. Cleaning PPE Needed: Lab Coat, Gloves, Safety Goggles/Glasses Supplies Needed: Apiezon M Vacuum grease, Hexanes Carefully disassemble the system (FRAGILE!) and clean with hexanes, disposing of it in normal organic waste. If necessary soak glass manifold in base bath (isopropanol with added potassium hydroxide). Cleaning Re-grease joints with Apiezon M Vacuum grease (orange t