大桑菊合剂对小鼠迟发型变态反应性肝损伤的影响.docVIP

大桑菊合剂对小鼠迟发型变态反应性肝损伤的影响 　　摘要：目的 观察大桑菊合剂对小鼠迟发型变态反应性肝损伤的影响，探讨其抗炎免疫的作用机制。方法 采用腹腔注射环磷酰胺处理后，予2，4，6-三硝基氯苯2次外涂腹部皮肤致敏，再经皮肝穿刺攻击造模。实验小鼠随机分为空白组、模型组和中药高、中、低剂量组。中药高、中、低剂量组给予大桑菊合剂灌胃，每日1次，连续7 d。ELISA检测小鼠血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）和肿瘤坏死因子-α（TNF-α）的水平，HE染色观察肝脏组织病理形态。结果 与正常组比较，模型组AST、ALT和TNF-α水平显著升高（P0.01）；与模型组比较，中药各剂量组ALT、AST和TNF-α水平显著降低（P0.05，P0.01，P0.001）；中药各剂量组较模型组均能减轻肝组织局部炎症浸润与水肿。结论 大桑菊合剂对小鼠迟发型变态反应性肝损伤具有保护作用。 　　关键词：大桑菊合剂；迟发型变态反应；肝损伤；小鼠 　　DOI：10.3969/j.issn.1005-5304.2016.07.019 　　中图分类号：R285.5 文献标识码：A 文章编号：1005-5304（2016）07-0074-03 　　Protective Effects of Dasangju Mixture on Liver Injury Induced by Delayed-type Hypersensitivity in Mice XU Jia-hui1， 2， HUANG Xue-jun1， ZENG Qiao-huang1， LAN Yan-jiao1， ZHANG Xiao-lu1， YU De-zhao2 （1. The Second Provincial Hospital of Traditional Chinese Medicine， Guangzhou 510000， China； 2. Guangzhou University of Chinese Medicine， Guangzhou 510000， China） 　　Abstract： Objective To investigate the protective effects of Dasangju Mixture on the liver injury induced by delayed-type hypersensitivity in mice； To discuss its mechanism of anti-inflammatory-immunity. Methods After enterocoelia intravenous injection of cyclophosphamide was used， 2，4，6-picryl chloride was used twice for exterior coating for skin of abdomen to cause sensation of skin. Then percutaneous transhepatic puncture was used to attack for modeling. Male Kunming mice were randomly divided into blank group， model group， high-， medium- and low-dose treatment groups. The high-， medium- and low-dose treatment groups were given Dasangju Mixture for gavage， once a day for 7 days. ELISA was applied to determine the serum levels of aspartate aminotransferase （AST）， alamine aminotransferase （ALT） and tumor necrosis factor （TNF）-α. Pathological features of liver tissue were observed by HE staining. Results Compared with the blank group， the levels of AST， ALT and TNF-α in the model group increased significantly （P0.05）； Compared with the model group， the levels of AST， ALT and TNF-α in