基于rDNAITS序列Alternar_省略_关系及A_leucanthem解剖.pdf

25 2 184~191, 2006 Mycosystema rDNA ITS A. leucanthemi Alternaria 1 2 1* 2 1 1 , 271018 2 , 100080 Alternaria, Stemphylium, Ulocladium, Nimbya, Embellisia 5.8S/ITS GenBank Pleospora, Lewia Neighbor-joining / 5.8SrDNA ITS ITS1 ITS2 5.8S/ITS Alternaria Ulocladium Nimbya Embellisia Stemphylium Stemphylium rDNA Alternaria leucanthemi Alternaria Stemphylium spp. Bipolaris spp. Pleospora Q939.5 A 1672-6472 2006 02-0184-0191 Phylogenetic relationships of Alternaria and related genera and taxonomic status of A. leucanthemi inferred from ITS rDNA sequence analysis ZHAO Guo-Zhu1, 2 ZHANG Tian-Yu1* CAO Ai-Xin2 WANG Hong-Kai1 (1. Department of Plant Pathology, Shandong Agricultural University, Taian 271018) (2. Key Laboratory of Systematic Mycology Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080) ABSTRACT: In order to further elucidate the relationships among Alternaria, Ulocladium, Stemphylium, Nimbya and Embellisia and their teleomorphic Pleospora and Lewia, nuclear internal transcribed spacer (ITS) and 5.8SrDNA from these genera/species are determined and compared. Alignment of the 5.8S/ITS of the tested isolates shows that 5.8SrDNA are highly conserved. The ITS sequences, especially ITS1, are very variable in both base composition and length. Phylogenetic analysis of the 5.8S/ITS sequence data sets by NJ (Neighbor-joining) method clearly separated these tested fungi on generic level. The results indicate that Alternaria spp., Ulocladium spp., Nimbya spp. and Embellisia spp. are placed together in a large * (Corresponding author) E-mail tyzhang@sdau.edu.cn 2005-10-26 2005-11-23 915 2 rDNA ITS Alternaria 185 A. leucanthemi Alternaria-complex clade and are closely related, they are relatively differentiated to some extent; Stemphylium spp. are clustered in a distant clade and phylogenetically distinct from the other four genera, showing a relative independent evolution. The anamorphic Stemphylium spp. and its teleomorphic Pleospo