特异乳酸杆菌对人直肠癌裸鼠异种移植瘤细胞增殖的影响.docVIP

特异乳酸杆菌对人直肠癌裸鼠异种移植瘤细胞增殖的影响 　　[摘要]目的 探讨特异乳酸杆菌（Lb-f）对人直肠癌裸鼠异种移植瘤模型癌细胞增殖的抑制作用。方法 采用小鼠背部皮下接种人直肠癌系（Colo320）细胞的方法建立裸鼠异种移植瘤模型，并随机分为五组，Ⅰ组作为模型对照组；Ⅱ～Ⅳ组分别以180、360、720 mg/kg的Lb-f灌胃，1次/d，灌胃2周。Ⅴ组腹腔注射化疗剂环磷酰胺100 mg/kg，1次/d，共计2周。利用MTT比色法和台盼蓝计数法测定各组的细胞增殖相对抑制率及生长抑制率，并取肿瘤组织，计算瘤重抑制率以及肿瘤生长抑制率，采用ELISA试剂盒测定Bax、Bcl-2、Caspase-3含量。结果 随着干预时间的延长，Colo 320瘤块体积有明显变化（P0.01），且在开始的48 h内，体积增大不明显，48 h之后，体积增大情况较为明显。体外实验结果显示，各组的细胞增殖相对抑制率和生长抑制率比较，差异有统计学意义（P0.01）；空白对照组和溶媒对照组的细胞增殖相对抑制率和生长抑制率比较，差异无统计学意义；其他组均较前两组高，且阳性药对照组为最高，其次是Lb-f 90组、Lb-f 60组、Lb-f 30组，各组之间的差异均有统计学意义（P0.01或P0.05）。在体实验结果显示，各组的细胞瘤重抑制率、肿瘤生长抑制率、肿瘤组织Bax含量、Bcl-2含量、Bcl-2/Bax、Caspase-3比较，差异有统计学意义（P0.01或P0.05）；细胞瘤重抑制率、肿瘤生长抑制率、肿瘤组织Bax含量按顺序由低到高分别是Ⅰ组、Ⅱ组、Ⅲ组、Ⅳ组、Ⅴ组，Bcl-2含量、Bcl-2/Bax、Caspase-3按顺序由低到高分别是Ⅴ组、Ⅳ组、Ⅲ组、Ⅱ组、Ⅰ组。结论 Lb-f可调节Bcl-2/Bax平衡，从而起到抑制肿瘤生长的作用，且呈剂量依赖性。 　　[关键词]直肠癌；裸鼠；异种移植瘤；特异乳酸菌 　　[中图分类号] R735.3+7 [文献标识码] A [文章编号] 1674-4721（2016）07（b）-0007-04 　　[Abstract]Oobjective To explore the inhibiting effect of Lactobacillus fermentum （Lb-f） to cancer cells proliferation of human colorectal cancer in nude mouse of xenograft tumor models.Methods The xenograft tumor models of human colorectal cancer in nude mouse were prepared by back subcutaneous injecting Colo320 in mice，and all the models were randomly divided into 5 group，Group Ⅰ was the control group，Group Ⅱ-Ⅳ were gavaged with Lb-f of 180，360，720 mg/kg respectively，one time every day for two weeks，and Group Ⅴ was given intraperitoneal chemotherapy cyclophosphamide of 100 mg/kg，one time every day for two weeks.Cell proliferation inhibition rate and growth inhibition rate among each group were detected using MTT colorimetry and trypan blue counting method.Further more，tumor tissues were extracted to calculate tumor inhibition rate and growth inhibition rate，and the content of Bax，Bcl-2，Caspase-3 was detected by ELISA kit.Results With the extension of intervention time，the volume of Colo320 tumor block was significantly changed （P 　　[Key words]Colorectal cancer；Nude mice；Xenograft tumor；Lactobacillus