非生物诱导子茉莉酸甲酯和水杨酸对半夏悬浮细胞中生物碱代谢的影响.docVIP

非生物诱导子茉莉酸甲酯和水杨酸对半夏悬浮细胞中生物碱代谢的影响 　　摘要：目的 研究非生物诱导子茉莉酸甲酯（MeJA）和水杨酸（SA）对半夏悬浮细胞系生物碱含量及相关酶活性的影响。方法 以叶柄来源的愈伤组织建立半夏悬浮细胞系，调查不同培养时间、MeJA和SA浓度对细胞中生物碱含量的影响，并分析生物碱合成相关酶（IMP脱氢酶、sAMP合成酶）的活性。结果 悬浮培养21 d时，悬浮细胞干重（DW）为培养前9倍，且生物碱总量为培养前3倍。MeJA和SA处理均能显著促进生物碱在半夏悬浮细胞中累积。150 ?mol/L MeJA处理组生物碱含量为对照组3.6倍，达4.7 mg/g DW；50 ?mol/L SA处理使悬浮细胞生物碱含量达到对照组的2.5倍。100 ?mol/L MeJA和50 ?mol/L SA处理使IMP脱氢酶比活力分别为对照组3.0、3.7倍，150 ?mol/L MeJA和100 ?mol/L SA处理使sAMP合成酶比活力分别为对照组2.6、4.4倍。结论 添加适量外源诱导子MeJA和SA能促进半夏悬浮细胞中生物碱的累积。 　　关键词：半夏；悬浮细胞；生物碱；茉莉酸甲酯；水杨酸 　　DOI：10.3969/j.issn.1005-5304.2017.01.021 　　中图分类号：R282.6 文献标识码：A 文章编号：1005-5304（2017）01-0087-04 　　Effects of Abiotic Elicitors MeJA and SA on Alkaloids Accumulation and Related Enzymes Metabolism in Pinellia ternata Suspension Cell Cultures DUAN Yong-bo， LU Fang， CUI Ting-ting， ZHAO Feng-lan， TENG Jing-tong， SHENG Wei， ZHANG Ai-min， XUE Jian-ping （Key Laboratory of Resource Plant Biology of Anhui Province， College of Life Sciences， Huaibei Normal University， Huaibei 235000， China） 　　Abstract： Objective To study the effects of abiotic elicitors methyl jasmonate （MeJA） and salicylic acid （SA） on the alkaloids accumulation and related enzymes metabolism in Pinellia ternata suspension cell cultures. Methods Using the leaf petioles-derived suspension cell cultures as the study object， the culture duration， concentrations of MeJA and SA were determined to get the optimal alkaloids accumulation， and the activities of metabolic enzymes IMP dehydrogenase and sAMP synthase were also measured. Results A 9-fold of dried biomass and a 3-fold of alkaloids accumulation were observed in P. ternata suspension cell cultures after culture for 21 d. Both MeJA and SA could significantly promote the accumulation of alkaloids in P. ternata suspension cells. 150 ?mol/L MeJA enhanced alkaloids content （4.7 mg/g DW） by 3.6 folds in comparison with control group， whereas 50 ?mol/L SA showed a 2.5-fold increase. Meanwhile， 100 ?mol/L MeJA and 50 ?mol/L SA promoted the increase in IMP dehy