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FOR IACUC OFFICE USE ONLY PROTOCOL #: AMENDMENT # AMENDMENT APPROVAL DATE: Tufts University Tufts Medical Center and the Human Nutrition Research Center on Aging Institutional Animal Care and Use Committee (IACUC) Telephone: 617-636-4109 Email: iacuc-office@ Website: /iacuc/ ANIMAL USE PROTOCOL AMENDMENT Amendments to protocols require Institutional Animal Care and Use Committee (IACUC) review and approval prior to initiation. The IACUC reserves the right to determine whether proposed changes require more information, full committee review, or submission of a new protocol. When submitting an amendment, the Principal Investigator is required to review all of the details of the original protocol to assure the IACUC that all un-amended details remain identical to the original protocol. Please note that certain changes to protocols may affect other aspects of the protocol. Those changes also need to be reflected in this amendment. I. GENERAL INFORMATION PRINCIPAL INVESTIGATOR: DEGREE(S): ACADEMIC POSITION/TITLE: DEPARTMENT: E-MAIL ADDRESS: MAILING ADDRESS: DIRECT PHONE #: EMERGENCY PHONE # LABORATORY MANAGER or PRIMARY CONTACT: DEGREE(S): E-MAIL ADDRESS: DIRECT PHONE #: EMERGENCY PHONE # PROTOCOL TITLE: PROPOSED MODIFICATIONS For applicable checkboxes, double-click on the box and then select “checked” to mark, and then complete the relevant sections of the amendment form to describe changes or additions to your original protocol. Not all sections in the amendment form are relevant for each type modification. Additional animals needed OR Change in Category New species to be used New procedure OR Change in procedure Change in location None of the above II. VERIFICATION OF REGULATORY APPROVALS Please check all that correspond to this IACUC protocol. Double-click on a box and then select “checked” to mark your selection. Note that the Principal Investigator is responsible for ensuring that the appropriate permits a