supplementarytable1thepublishedinvitrotransdiffe.docVIP

Supplementary Table 1: The published in vitro transdifferentiation reports (Species) Starting cell Desired cell (%) Gene Delivery System Defined genes Niche Final evaluation Main Results Ref Myoblast Mouse Fibroblast Myoblast (0.1-50%) depend on cell type pCMV.neo or pLNSAL MyoD - ICC analysis of myosin. First report on transdifferentiation. 1 Fibroblast, Chondroblast, Smooth muscle cell, RPE cell ND Retroviral (amphoteric retroviruses) MyoD - IF analysis of Des, α-actinin, titin, Neb, α-actin, troponin-I, tropomyosin, MHC, MLC, myomesin. Conversion of different cell types into stable myoblasts. 2 Fibroblast Cardiomyocyte (30%) Retroviral Gata4, Mef2c, Tbx5 - FACS analysis of cTnT; RT-PCR analysis of Myh6, Actc1, Actn2, Nppa; IF analysis of cTnT, α-actinin, Anf. First report on fibroblast-derived functional cardiomyocytes. 3 Fibroblast Cardiomyocyte (40%) Retroviral (inducible lentiviruses) Oct4, Sox2, Klf4 and c-Myc (c-Myc is not necessary for MEFs) - Screening beating patches; IF analysis of Flk1, Nkx2.5, Gata4, Myosin, cTnT, Connexin-43, α-actinin, MLC-2a; FACS analysis of Nanog, SSEA-1, Flk-1, Nkx2.5, cTnT; Electrophysiological analysis; Epigenetic analysis. First report on generation of beating cardiomyocytes from fibroblasts by initial activation epigenetic phase and optimization of appropriate niche. 4 Blood cell lineage Human Fibroblast CD45+ in neonatal fibroblast (24%), CD45+ in adult (38%) Lentiviral OCT4 - FACS analysis of CD45; Global gene expression analysis (compare with peripheral blood MNC and UCB derived CD34+); After cytokine treatment: FACS analysis of hematopoietic lineages markers; CFU Assay; Transplantation; Macrophage activity assay. Forced expression of OCT4 in fibroblasts lead to multipotent hematopoietic progenitors of myeloid, erythroid and megakaryocyte lineage. 5 Hepatocyte Mouse Exocrine cell line ND Non-Viral (plasmid, pCDNA) C/EBP β - IF analysis of Trf, G6P, Ttr, amylase. C/EBPβ is sufficient and cr