syllabusforbio126l(spring2006)dr.pratibhasaxena.docVIP

SYLLABUS FOR BIO 126 L (Spring 2006) Dr. Pratibha Saxena You need to be either co-registered in BIO 226R/N or have taken their equivalent earlier Class website: /psaxena/ SCHEDULE OF LAB EXERCISES WEEK STARTING LAB Jan 23 Lab check-in Guidelines for success Cleanliness and safety Jan 30 Lab 1: Use of the light microscope (video-1) Feb 6 Ubiquity of microorganisms Appendix A: Sterilization Lab 2: Pure culture techniques (video-2, 2 clips) Turn in practice streak plate Feb 13 Lab 3: Selection and differentiation Graded Streak Plate (20 points) Feb 20 Lab 4: Dilution and plating (video-4, 3 clips) Colorimetry and turbidimetry Feb 27 Lab 5: Identification of organisms (video-5, 5 clips) Mixed culture unknown (60 points) March 6 Labs 6 / or 7: Bacterial growth I II March 13 Spring Break (Have Fun!) March 20 Growth Curve Lab (Graphing) Lab 8: Enrichment culture Review March 27 Lab 9: Phenotypic variation, isolation and characterization of bacterial mutants Review March 31 EXAM I Mon Labs Welch 2.224 (Fri) Tues Labs Welch 3.502 5:30p.m. - 7:30p.m. Covers Labs 1-6 Wed Labs Welch 1.308 Th Labs Welch 1.316 April 3 Lab 10: Pathogenic bacteria Unknown mixture (90 points) April 10 Lab 11: Phage and DNA (video-11) April 17 Lab 12: Bacterial Conjugation April 24 Lab 13: Serology Graded Streak Plate (20 points) Review April 28 EXAM II Mon Labs Welch 2.224 (Fri) Tues Labs Welch 3.502 5:30p.m. - 7:30p.m. Covers Labs 8-13 Wed Labs Welch 1.308 Th Labs Welch 1.316 REMEMBER TO CLEAN OUT YOUR INCUBATOR SPACE HALL LOCKERS BY FRI April 28 (Worth 20 points of technique/participation) SUPPLIES AND EQUIPMENT REQUIRED FOR EACH STUDENT 1. Laboratory manual: available at Speedway Printing in Dobie Mall 2. Lock for hall locker (to be purchased separately) 3. Inoculating wire holder with knurled chuck (wire will be supplied) 4. Pre cleaned