Protoplastculture（原生质体培养）.ppt

Chapter 3Protoplast culture Somatic hybridization Part 1 Protoplast culture Part 2 Somatic hybridization Wen-Wu Guo（郭文武教授） Part 1: Protoplast culture （原生质体培养） Definition of Protoplast Protoplast (原生质体): a naked cell without cell wall Sub-protoplast (亚原生质体)：incomplete protoplast with or without nucleus Nucleo-plast (核质体)：nuclear protoplast with little cytoplasm (由原生质膜和薄层细胞质包围细胞核形成的小原生质体，即小原生质体) (Miniprotoplast) Micro-protoplast（微原生质体）: protoplast with one or a few chromosomes Cytoplast (胞质体): enucleated protoplast (不含细胞核而仅含部分胞质的原生质体) Cytoplast isolation in Citrus Xu et al., 2006 Progr