FQ―PCR检测丹江口地区解脲支原体、沙眼衣原体的五年回顾性研究.docVIP

FQ―PCR检测丹江口地区解脲支原体、沙眼衣原体的五年回顾性研究 　　摘要：目的 分析研究实时荧光定量聚合酶链式反应（FQ-PCR）对解脲支原体、沙眼衣原体的检测结果，以了解本地区在不同性别及年龄段的感染情况。方法 选取1000例疑似非淋菌性尿道炎患者，均采取FQ-PCR进行检测，统计分析不同男女性别及年龄段的阳性检出率。结果 UU和Ct的检出率为44.50%，其中在女性患者中的检出率（55.31%）要明显高于男性（35.58%）（P0.05）。18～40岁患者的阳性率（81.35%）显著高于年龄大于40岁患者（18.65%），差异显著（P0.05）。结论 FQ-PCR对UU、Ct的检出率较高，本地区以女性患者及18～40岁年龄段的检出率较高，应加大对上述人群的监管力度。 　　?P键词：FQ-PCR；解脲支原体；沙眼衣原体 　　A Retrospective Study of FQ-PCR in Danjiangkou Area of Ureaplasma Urealyticum and Chlamydia Trachomatis in Five Years 　　DENG Li1，SONG Dan1，FANG Zheng-chun2 　　（1.Department of Clinical Laboratory，The First Hospital of Danjiangkou City，Danjiangkou 442700，Hubei，China；2.Department of Anesthesiology，The First Hospital of Danjiangkou City，Danjiangkou 442700，Hubei，China） 　　Abstract：Objective Analysis of real-time fluorescence quantitative polymerase chain reaction （FQ-PCR）of Ureaplasma urealyticum and Chlamydia trachomatis detection results， in order to understand the region in different gender and age of infection. Methods A total of 1000 cases of suspected patients with nongonococcal urethritis， FQ-PCR detection. Statistical analysis of different gender and age of the positive rate. Results The detection rate of UU and Ct was 44.50%， among which the detection rate （55.31%） in female patients was significantly higher than that in males（35.58%）（P0.05）. The positive rate （81.35%） of 18-40 years old patients was significantly higher than that in patients older than 40 years （18.65%）， and the difference was significant （P0.05）. Conclusion The detection rate of UU in FQ-PCR and Ct is higher， the detection rate of female patients in the local area and the age of 18～40 years old is higher， and the supervision of these people should be increased. 　　Key words：FQ-PCR；Ureaplasma urealyticum；Chlamydia trachomatis 　　在临床中，非淋菌性尿道炎（nongonococcalurethritis，NGU）属于常见性传播疾病[1-2]。该病在欧美等发达国家、地区的发病率居首位，且自2001年以来我国报道NGU发病率已经超过淋病，在全国监测8种性传播疾病中居首位。NGU及并发症，如睾丸炎、前列腺炎、盆腔炎、不孕不育等对人类的健康构成极大的危害。因此需要对该症进行及时诊断，以免耽误治疗[3-5]。而据统计在NGU中40