CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli.pdfVIP
- 1、本文档共10页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli.pdf
Metabolic Engineering 41 (2017) 1–10
Contents lists available at ScienceDirect
Metabolic Engineering
journal homepage: /locate/ymben
CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli
Liya Lianga, Rongming Liua, Andrew D. Garstb, Thomas Leec, Violeta Sànchez i. Noguéd, Gregg T. Beckhamd, Ryan T. Gilla,?
a Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, Boulder, CO 80303, United States b Muse Biotechnology Inc., 4001 Discovery Dr., Boulder, CO 80303, United States c Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO 80309 United States d National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, United States
MARK
ARTICLE INFO
Keywords: Isopropanol CRISPR EnAbled Trackable genome Engineering RBS library Engineered Escherichia coli Proteomic analysis
ABSTRACT
Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, construction, and testing of production strains. Here, we report on the use of such capabilities to construct and test 903 di?erent variants of the isopropanol production pathway in Escherichia coli. We ?rst constructed variants to explore the e?ect of codon optimization, copy number, and translation initiation rates on isopropanol production. The best strain (PA06) produced isopropanol at titers of 17.5 g/L, with a yield of 0.62 (mol/mol), and maximum productivity of 0.40 g/L/h. We next integrated the isopropanol synthetic pathway into the genome and then used the CRISPR EnAbled Trackable genome Engineering (CREATE) strategy to generate an additional 640 individual RBS library variants for further evaluation. After testing each of these variants, we constructed a combinatorial library containing 256 total variants from four di?erent RBS l
您可能关注的文档
- A case study on the growth of Lanice conchilega (Pallas, 1766) aggregations and their ecosystem engineering impact on sedimentary processes.pdf
- A new model to predict diffusive self-heating during composting incorporating the reaction engineering approach (REA) framework.pdf
- A novel comby scaffold with improved mechanical strength for bone tissue engineering.pdf
- A RFBSE model for capturing engineers’ useful knowledge and experience during the design process.pdf
- A survey of the use of crowdsourcing in software engineering.pdf
- Advances in assessing myotonia Can sensor-engineered glove have a role.pdf
- Alleviation of metabolic bottleneck by combinatorial engineering enhanced astaxanthin synthesis in Saccharomyces cerevisiae.pdf
- Anisotropic Raman scattering and mobility in monolayer 1Td-ReS2 controlled by strain engineering.pdf
- Bandgap engineering of (AgIn)xZn2(1x)S2 quantum dot photosensitizers for photocatalytic H2 generation.pdf
- Bio-based materials with novel characteristics for tissue engineering applications – A review.pdf
最近下载
- 肝脏常见疾病超声诊断.pptx VIP
- 化工设备机械基础课件-梁的弯曲.ppt VIP
- 新版配电网规划设计技术导则QGDW 10738—2020.pdf VIP
- 质保期服务承诺及维保.doc
- DL/T5437-2009《火力发电建设工程启动试运及验收规程》.pdf
- Chapter 4 Intercultural Communication Barriers Chapter 3 Culture’s Influence on Perception 大学英语跨文化交际 教学课件.ppt VIP
- 做酸奶ppt课件完整版.ppt
- 拉伸压缩与剪切扭转课件.ppt VIP
- 天然气井溢流井喷预防与压井现场处理技术.pptx
- 4008S血液滤过机规范操作流程.ppt VIP
文档评论(0)