PCR—RFLP基因分型方法.pptVIP

PCR-RFLP 基因分型方法 关于序列查找、引物设计和酶切位点分析 http://www.lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/ 定点诱变引物设计（二） 5-ggattcagaatgattctctccaagaaaacatcctttttggatg-3 3-cctaagtcttactaagagaggttcttttgtaggaaaaacctac-5 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 酶切位点分析 新建窗口 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 酶切位点分析 输入序列，注意粘贴的序列不能有空格和段落标记符号 大写突变位点以便于辨认 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 酶切位点分析 选择菜单 Analyze Restriction maping Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 酶切位点分析 酶谱选择 点击确定 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. * * Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 概念 聚合酶链式反应连接的限制性片段长度多态性分析 限制性片段长度多态性(restrition fragment length polymophism，RFLP): 是指由限制性酶切位点间的插入﹑缺失﹑重排或 点突变所引起的基因型间限制性片段长度的变异。 PCR-RFLP: 是在 PCR 和 DNA 序列分析基础上产生的 RFLP 技术。该方法是通过 PCR 扩增一段 DNA 片段，然后再选择适当的限制性内切酶，消化 PCR 产物，经电泳，可得到有特异性的电泳谱带，从而达到鉴定不同基因型的目的。 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 概念 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 概念 确定基因序列 设计引物 选择内切酶 电 泳 基 因 型 Step by step Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 基因序列查找 http://www.genomatix.de/ /Genbank /sprot/ Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright 2004-2011 Aspose Pty Ltd. 基因序列查找 选择 gene 输入基因名称 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile . Copyright