5氟尿嘧啶控释植入剂在小鼠肿瘤组织的分布.pdfVIP

Central South Pharmacy. September 2012, Vol. 10 No. 9 5- * , 710061 5- 5- RP-HPLC 97.5% 104.0% 50/50 75/25 5- 3.79 2.83 μg ? g AUC 2 PLGA 50/50 81.9% 88.3% 41.15 43.50 μg ? g 5- PLGA 75/25 5-Fu RSD 2.0% d 5-Fu 5-Fu 0.5 15.4 μg ? g RSD 7.6% tmax 9 15 d Cmax 5- 5R944.9 doi:10.3969/j.issn. 1672.2981.2012.09.001 A 1672-2981(2012)09-0641-03 Distribution of 5-fluorouracil controlled release implant in tumor bearing mice ZHOU Ming-yao, REN Xiao-jiao, LI Han-wen, LU Wen * (School of Medicine, Xi’an Jiaotong University, Xi’an 710061 ) Abstract: Objective To establish an assay of 5-fluorouracil (5-Fu) in tumor bearing mice, and to investigate the distribution of 5-Fu controlled release implant in the tumor. Methods The 5-Fu controlled release implant prepared by PLGA 50/50 and PLGA75/25 as carriers was inserted around the tumor. The concentrations of 5-Fu in the tumor were determined by RPHPLC. Results The linearity of 5-Fu in the tumor was 0.5 15.4 μg ? g . The accuracy and the extraction recovery were 97.5% 104.0% and 81.9% 88.3%. The intra-day and inter-day RSD were less than 2.0% and 7.6%, respectively. The tmax , Cmax and AUC for PLGA 50/50 implant were 9 d, 3.79 μg ? g 1and 41.15 (μg ? g ) d, respectively. The tmax , Cmax and AUC for PLGA 75/25 implant were 15 d, 2.83 μg ? g and 43.50 ( μg ? g ) d, respectively. Conclusion The method is reliable in the study of distribution of 5-Fu in tumor bearing mice. The 5-Fu controlled release implant prepared with 2 kinds of polymers could extend the duration of drugs in the tumor. Key words: 5-fluorouracil; controlled release implant; HPLC; tumor tissue distribution 5- (5-fluorouracil 5-Fu) [1-2] 5-Fu 5-Fu 5-Fu 5-Fu [3] [5-8] [4] 2 1 HPLC LC-10ATvp SPD-10Avp SHIMADZU ANASTAR 5-Fu KDC-16H SB-5200DTD 5301 Eppendorf QL-901 LGJ-10D 5-Fu5-Fu 99.0% 5- 5-Bru99.8% 5-Fu 10 mm 10 mm 1 mm 7.2 mg PLGA50/50 2011K12-03-04 Tel 029 TelE-mail zhoumingyao825@stu.xjtu.edu.cnE-mail lvlu2004@mail.xjtu.edu.cn