GUS基因概述.pdfVIP

17 3 V o l117 N o 13 JOU RNAL O F GU AN GX I A GR ICU L TU RAL U N IV ER S IT Y 1998 9 Sep t. 1998 GUS Ξ 112 (1 , 530005; 2 , 530005) GU S (Β , () GU S (Β Q 789 ) , GU S ); , ; A M in irev iew on GUS Gene GU S W ei L ili1 Fan M iao ji1 Chen L im ei2 (1 Co llege of B io techno logy & Suga r Indu st ry Eng ineering; 2 T he Key L abo ra to ry of A g ric. of M o lecu la r Genet ics of the Ch inese M in ist ry of A g ric. , Guangx i U n iv. , N ann ing 530005) Abstract GU S (Β g lucu ron ida se) gene a s one k ind of repo rt gene in gene fu2 sion sy stem w a s cloned from E scherich ia coli. It have been u sed in m o lecu la r genet2 ics resea rch such a s gene con t ro l and gene exp ression of bacteria, p lan t s even an i2 m a ls. T h is p ap er w ill b riefly recomm end the cha racter and the chem ica l ana lysis of GU S, the clon ing and app lying of GU S gene. Key words GU S (Β g lucu ron ida se) gene; repo rt gene; enzym e (CA T ) (O CS) , , , 6, Β (A PH 3 II, N PT II) , , (L azZ) (NO S) , , Ξ ( 9618002) : , 1955 , : 1997 20 29 ? 1994-2010 China Academic Journal Electronic Publishing House. All rights reserved. 300 ; 17 ; ;; , ,Β 1 GU S 1961 Stoeber Β ,Β 68 200 , , GU S , [1 ] GU S ED TA ( pH 512 810, pH 413 50 2 h , GU S 4 ), Pka8 9, , GU S , pH pH GU S [3] (EC 3 2 1 31) [1], Β (GU S) ,, Β DTT ( ) C u2+ , Zn2+ ), pH 815 50% [2], 100 1 000 4 , (7 4 , , ( ) , GU S (X g luC ) 9 4 3 (M U G ) 5 (R eG) [3] , [3] , (GU S) (X g luC ) , [4] R eG , 560 nm , , pH 43 GU S , GU S 590 nm , , , 2 GU S GU S u idA , gu sA ( u idA ) DNA , 1 809 bp , K12 N ovel [5 7 ] J effe r so n [8 ] u idA u idA , GU S , : pRA J 255 ? 1994-2010 China Academic Journal Electronic Publishing House. All rights reserved. 3 : GU S 301 1187 kb EcoR 1 P st1 GU S , p EM BL 9 pRA J 260 pRA J 255, EcoR 1 P st1 , P st1 p EM BL 9 P st1 GU S pL 6GU Sc [9] GU S pB I101 ,[ 10, 11 ] , , GU S pB IN 19 , pLA FR GU S , GU S , , , , PK2 , , pB I13311 rbcS [11 ] pB I101. 1 T n5 gu sA :[ 12 ] Tn5 GU S , , GU S , GU