simulationofantioxidativeprocessatbiomembrane.pptVIP

* simulation of antioxidative process at biomembrane simulation of antioxidative process at biomembrane oil/water interface (water) ( oil ) Ideal model interface of two immiscible electrolytes biomembrane water droplets with antioxidant free radicals R reducibility: ＞ R M+ M ② ① ② R R R+ emulsification ③ The reaction ③ is prior to the reaction ②. intercellular fluid lipid bilayer Oxidation of M is inhibited. key words 1. antioxidant a molecule capable of inhibiting the oxidation of other molecules 2. Chlorogenic acid an important biosynthetic antioxidant It also is one of the phenols found in coffee, bamboo, as well as many other plants. imagination Fc GC aqueous phase oil phase Fc+ antioxidant E / V I / A oil phase aqueous solution simulation aim The aim is to get the basic data of the reactions in both phases. CV of Fc in NB solution solvent: NB reactant: 0.4 mM Fc electrolytic salt: 200 mM TBACl composition of solution RE CE WE WE ---- GC disc (3mm) CE ---- Pt coil (0.5mm) RE ---- Ag/Ag+ ΔE = EAg/Ag+ - EAg/AgCl= 0.491 V CV of Fc in NB solution ΔE = EAg/Ag+ - EAg/AgCl= 0.491 V Average decreasing ratio of Ipa is 18.0%. Fc Fc＋ Fc＋ Fc Oxidation products was adsorbed on the WE. Organic film on the WE surface was observed after every CV detections.  redox-process diffusion controlled CV of coffee beans aqueous solution solvent: water reactant: 1mg coffee / 1ml solution phosphate buffer solution pH = 7.0 composition of solution RE CE WE WE ---- GC disc (3mm) CE ---- Pt coil (0.5mm) RE ---- Ag/AgCl Food Chemistry 82 (2003) 501–512 Paul A. Kilmartin*, Chyong F. Hsu CV of coffee beans aqueous solution Average decreasing ratio of Ipa is 35.5%. Oxidation products was adsorbed on the WE. Organic film on the WE surface was observed after every CV detections.  oxidation process diffusion controlled reduction process adsorption controlled *