bs-8093 菌株的鉴定及其对青枯雷尔氏菌的致弱作用.docVIP

Bs-8093菌株的鉴定及其对青枯雷尔氏菌的致弱作用( 周先治1，2，张青文2，林营志1，朱育菁1，刘波1* （1.福建省农业科学院生物技术研究所， 福州 350003；2.中国农业大学农学与生物技术学院，北京 100094） 摘要：Rs-T.1.3-010702-01、Rs-T.1.3-010702-25、Rs-T.1.3-010702-27和Rs-E.1.3-010719-03；第二类菌株易致弱，包括菌株Rs-T.1.3-010702-07、Rs-T.1.3-010702-14、Rs-T.1.3-010702-17、Rs-T.1.3-010702-18、Rs-T.1.3-010702-19、Rs-T.1.3-010702-20、Rs-T.1.3-010702-21、Rs-T.1.2-010618-07、Rs-C.1.4-010704-02、Rs-G.1.4-010704-01、Rs-G.1.4-010704-02和Rs-E.1.3-010719-02；Bs-8093菌株对第三类菌株的致弱能力处于第一类和第二类之间，包括菌株Rs-E.1.3-010719-04。 关键词：Bs-8093菌株，鉴定，致弱作用 Identification of Strain Bs-8093 and Its Attenuation Effects on Virulent Strains of Ralstonia solanacearum Zhou Xian-zhi，Zhang Qing-wen，Lin Ying-zhi，Zhu Yu-jing，Liu Bo (1. Biotechnology Research institute，Fujian Academy of Agricultural Sciences，Fuzhou 350003，China) Absract:The bacterium strain Bs-8093 isolated from the soil of tomato field. This strain was identified as Bacillus sphaericus according to its morphological, physiological and biochemical characteristics, Fatty acid chromatogram and 16S rDNA sequence analysis. The optimum temperature for the growth of the strain was 30-35℃. Bs-8093 has attenuation effects on virulent strain of R. solanacearum, according to the difference attenuation ability of strain Bs-8093 to 17strains R. solanacearum, the 17strains divided into 3 groups, the first group is difficult to attenuate, including strain Rs-T.1.3-010702-01, Rs-T.1.3-010702-25, Rs-T.1.3-010702-27, Rs-E.1.3-010719-03. The second group is easy to attenuate, including strain Rs-T.1.3-010702-07, Rs-T.1.3-010702-14, Rs-T.1.3-010702-17, Rs-T.1.3-010702-18, Rs-T.1.3-010702-19, Rs-T.1.3-010702-20, Rs-T.1.3-010702-21, Rs-T.1.2-010618-07, Rs-C.1.4-010704-02, Rs-G.1.4-010704-01, Rs-G.1.4-010704-02 and Rs-E.1.3-010719-02. The third group between the first and the second, including strain Rs-E.1.3-010719-04. Key words: bacterium strain Bs-8093; identification; attenuation effects 细菌性青枯病是一种由青枯雷尔氏菌（Ralstonia solanacearum）引起的毁灭性土传病害。青枯雷尔氏菌可危害几百种植物，寄主植物包括番茄、马铃薯、烟草、茄