影响油菜小孢子胚成苗的若干因素研究.pdfVIP

江西农业学报 2011 ， 23(9):54 -56 Acta A伊culturae Jian伊 影晌油菜小子包子胚成苗的若干因素研究 龙卫华，浦惠明，高建芹，胡茂龙，成存扣，张洁夫，陈松，陈新军，陈锋，顾慧 (江苏省农业科学院经济作物研究所，江苏南京 21∞14) 摘要川、袍子胚转化成苗是油菜高效小抱子培养体系的重妥环节。探索了基因型、激素、冷处理、琼脂糖浓度以及胚大 小等因素对油菜小袍子胚成苗率的影响，结果表明:采用一定措施可明显提高胚成笛率，获得更多的小抱子苗。不同基因型 材料的小袍子胚成苗率有明显不同，最高的胚成苗率可达42.9%;添加激素 GA 和提高培养基的综脂浓度至 1. 5% 可提高部 3 分基因型材料的成苗率;在4 吃下处理4 -7 d 也可提高成苗率;同一基因型内长度5 mm 的小抱子胚成苗率较高。 关键词:油菜:小抱子结养;胚成苗率;体系构建 中图分类号:5565.4 文献标识码:A 文章编号:1∞1 - 8581 (2011)09 -∞54-00 Study on Several Factors AtTecting Plantlet Formation from 如ficrlωpore Embryo of Brassica napω IρNG Wei-h础， PU H山 -rru鸣， GAO Jian-φn ， HU M皿-10鸣， QI Cun - kou , ZHANG Jie -fu , CHEN Song , CHEN Xin - jun , CHEN Feng , GU H时 (Institute of Economic Crof圃， Jiangsu Academy of Agricultural Scienc咽， Nanjing 21∞14 ， China) . Ah!此ract: The tr回回formation of rni臼回归陀 embry田 into plantlets w凶 an important step in 由e highly - efficient micros阳re cul- ture system of Brassica oopω ， many factors could afIect the rate of seedling formation from microspore embry咽， such as genotype , hor- mone，∞Ild tr唱atment ， ag缸ose concentration and embryo size , and 由ey were studied in this paper. 四e results showed that it could ob- viously illCrease the s臼d1ing ra忧 of embryos and get more micros阳陀 seedling自由rough adopt吨田Itne ml阅SUre8. The s帽d1ing rate of microspore embryl恼。f different genotype materials w圈。bviously different , and its maximum was 42.9% .ηle seedling rate of micro- spore emb巧。sof 田me genot川e materials could le increased by adding hormone GA3 and enhancing the a伊r ∞ncentration of culture u时ium to 1. 5%. Treating microspore embryos at 4 c for 4 -7 days al回四uld increase its s目d1ing rate. For the 田rne genotype ma- terial ，由.e microspore