采用卵子-胚胎移植技术分析研究家畜受精和早期发育.pdf

中国农业科技导报，2013，15(1)：65—70 Journal of Agricultural Science and Technology Egg-Embryo Transfer：An Analytical Tool for Vintage Experiments in Domestic Farm Animals Hunter R H F ．LI Xi．he。， (1．Institute for Reproductive Medicin，Hannover Vetednaf University，Hannover D一30559，Germany；2．Research Center for Animal Genetic Resources of Mongolia Plateau，Inner Mongolia University，Hohhot 010021，China；3．Inner Mongolia Saikexing Reproductive Biotechnology Co．Ltd．，Mengniu Dairy Research and Development Center，Hohhot O11517，China) Abstract：In order to clarify the fertilisation and early development or the progression of unfertilised eggs in the oviduct of domestic farm animals，four transplant studies are described in the study．① Pig eggs transplanted from ovulations induced during the luteal phase of the oestrous cycle were fertilised in the oviducts of inseminated oestrous animals．By contrast，pig eggs from oestrous donors became highly polyspermic when transplanted to the oviducts of animals force—mated during the luteal phase．② Pig embryos at the stage of hatched bfastoeysts(7 d and 8 d)could be transplanted successfuly to synchronous recipients and ful embryonic development be demonstrated between 19 d and 23 d．Thus，the exposed tropheetoderm of enlarging embryos could withstand the physical manipulation of recovery and transplantation，and the lifespan of corpora lutea in the unmated recipients could be prolonged after 7 d and 8 d transfered．③ Bovine oocytes aspirated from 2～6 mnl diameter Graafian follicles and matured in vitro were fertilised normaly in the oviducts of inseminated recipient heifers，demonstrating the potential of slaughterhouse ovaries for the generation of embryos．④ Transplanting equine eggs to a pig oviduct，in which egg descent to the uterus requires only 46—48 h，did not reveal a retarded progress of degenerating unfertilised horse eggs，suggesting the involvement of non—physical factors in equine embryo progression to the uterus．Prostaglandins of embryoni