稳定性同位素探针技术在土壤功能微生物原位鉴定的应用.pdfVIP

农业环境科学学报2012，31(1)：1-6 Journal of Agro-Environment Science 稳定性同位素探针技术在土壤功能微生物 原位鉴定的应用 蔺 中，孙礼勇，陈 昊，孙迎韬，白 婧，李永涛+ (华南农业大学资源环境学院，广州510642) 摘要：新近发展起来的稳定性同位素探针技术(SIP)与分子生物学方法结合，能够定向发掘复杂环境中参与特定生态过程的微生 物资源。是土壤功能微生物原位鉴定的有效手段，具有广阔的应用前景。其原理是环境样品中的功能微生物代谢同化同位索标记的 底物，通过对其生物标志物(即DNA、RNA、PLFA等)进行提取、分离、鉴定和比对分析。以此获取介导土壤物质转化和循环过程的 功能微生物的直接信息。本文在分别介绍SIP技术在土壤功能微生物原位鉴定过程中的各种前处理方法、生物标志物选择及后续 鉴定方法的基础上，综述了SIP技术在研究驱动土壤有机污染物生物降解、碳氮循环等过程的功能微生物原位鉴定的应用进展，展 望了SIP技术在土壤微生物基因组学方面的应用前景。 关键词：土壤；稳定性同位素探针技术；原位鉴定；功能微生物 中图分类号：S154．3 文献标志码：A 文章编号：1672—2043(2012)01-0001-06 Application of Stable Isotope Probing on／n-s／tu Identification of Soil Functional Microorganism LIN Zhong，SUN Li-yong，CHEN Hao，SUN Ying-tao，BAI Jing,LI Yong—tao‘ (College ofNatural Resources and Environment，South China A鲥cultural University，Guangzhou 510642，China) Abstract：Utilization of stable isotope probing(SIP)coupled with molecular biology method，can explore microorganisms flouI℃e responsible for specific process in natural environmenL and has a broad application prospects for soil functional microorganism／n situ identification．The principle of the SIP technique is鹬foHows．Functional microorganisms in soil samples．that metabolize the SIP-labeled substrates and assim- ilate SIP—labeled biomarkers，e．g．，DNA，RNA and PLFA，can be identified by extraction，separation and analysis of these SIP—labeled biomarkers，and then information of soil functional microorganisms responsible for transformation and cyclic process of substrate in soil call be obtained directly as a result．With introduction of pre—treatment technology，SIP-labeled biomarker selection,and subsequent microorgan- isms’identification method，this study illustrates the research progress ofSIP technique in in-situ identification offunctional microorgantisms involving in the soil organic pollutants degradation，carbon and nitrogen cycle．It will provide a comprehensive reference for the applic：at／on and exploration of SIP-based in-situ identification of soil microbial genomics in future