75.醋芫花指纹图谱----李林讲义.pdfVIP

醋芫花指纹图谱研究 李林 1，殷放宙 2，陆兔林 1，关洪月 2，蔡宝昌 2，3 1 （1 南京中医药大学药学院，江苏 南京，210023；2 江苏省中药炮制重点实验室，江苏 南京，210023；3 国家中医药管理局中药炮制标准重点研究室，江苏 南京，210023） 摘要 目的：对传统的指纹图谱进行改进，以区分醋芫花和生芫花。方法：收集了来源于 中国的10个批次的芫花，经过规范炮制成醋芫花，将这些样品在最优的液相条件下进行分析， 基于这些色谱数据，形成了包括11个共有峰的醋芫花指纹图谱共有模式，并且指认了其中5 个成分分别是芫花素、羟基芫花素、木犀草素、芹菜素、芫花酯甲。通过在样品中定量加入 外源性成分-黄芩素作为内标，以黄芩素峰为参比峰，利用本研究开发的改良距离系数法计 算待测样品与共有模式的相似度，结果：醋芫花相似度均高于0.9，生芫花均低于0.9。结论： 基于改良距离系数法开发的醋芫花指纹图谱可有效的区分醋芫花和生芫花，从而保证了临床 用药的安全，扩展了指纹图谱的应用领域。 关键词：芫花, 指纹图谱，相似度, 改良距离系数法 Research on Vinegared Flos Genkwa Fingerprint Abstract Objective ： Improving the traditional fingerprint method used to distinguish vinegared Flos Genkwa from crude Flos Genkwa. Methods：Ten batches of Flos Genkwa were collected in China, processed with vinegar through a standard method, and then analyzed under the optimum liquid phase. Based on the chromatographic data obtained, a common model of vinegar Flos Genkwa fingerprints, including 11 common peaks and the components genkwanin, hydroxygenkwanin, luteolin, apigenin, and yuanhuacin, was established. The peak of baicalein, an exogenous component added quantitatively to the samples as an internal standard, served as the reference peak. The similarity between the test samples and the common model was computed using the improved Euclidean distance method developed in this paper. Results ： The similarities between vinegared Flos Genkwa samples and the common model were higher than 0.9, whereas those between crude Flos Genkwa and the common model were lower 基金资助：国家科技部“重大新药创制”专项“中药饮片质量标准研究”（2012ZX09304005003）；江苏省“青蓝工程”优秀青年 骨干教师资助项目；江苏高校优势学科建设工程资助项目 作者简介：李林﹙1978-﹚，男，副教授，研究方向：中药炮制；E-m ail：lilin_med@163.com。 than 0.9. Conclusion： The proposed method thus effectively provides a clear distinction between vinegared and crude Flos Genkwa samples. The results of this study help ensure the safe clinical use of the plant and expand the application field of fingerprinting techno