夹层杯集菌离心涂片法检测抗酸杆菌对肺结核诊断的价值.docVIP

夹层杯集菌离心涂片法检测抗酸杆菌对肺结核诊断的价值 尚好珍，席秀娥，王金绳，高健彬，张斌，柴青峰 (新乡医学院第一附属医院结核内科，河南 卫辉453100) 摘要： 目的 探讨夹层杯集菌离心涂片法检测抗酸杆菌对肺结核诊断的意义。方法对614份痰标本同时进行夹层杯集菌离心涂片法与普通痰涂片法检测抗酸杆菌，比较2种方法的检出率。结果 夹层杯集菌离心涂片法检测抗酸杆菌阳性率为32．28％ ，明显高于普通涂片法镜检法的19．87％(P0．005)。结论 夹层杯集菌离心涂片法简单省时、阳性率高，且便于进行质量控制，是一种很有推广价值的检测方法。 关键词： 痰；抗酸杆菌；夹层杯集菌离心涂片；肺结核 中图分类号：R521 文献标识码：A 文章编号：1004-7239(2007)05—0493．02 The value of detection acid-fast bacilli by centrifuging the Liquored sputa and collecting bacteria in diagnosis of pulmonary tuberculosis SHANG Hao—zhen，XI Xiu—e，WANG Jin—sheng, etal (Department of Tuberculosis，the First Affiliated Hospital of Xinxiang Medical College，Weihui 453100，China) Abstract： Objective To explore the value of detection acid—fast bacilli by centrifuging the Liquored sputa and collecting bacteria in diagnosis of pulmonary tuberculosis．Methods: 614 sputum samples were detected by centrifuging the liquored sputa and collecting and normal smear method simultaneously．The detection rate was compared between two methods．Results: The positive rate of centrifuging the liquored sputa and collecting bacteria was 32．28％ ，it is significantly higher than the normal smear method (19．87％)．There was a significantly difference between two methods(P0．005)．Conclusion: The method of centrifuging the Liquored sputa and collecting bacteria is easy and can save time，the detection rate is high，and the quality control Can be easily done．It is value to be spreader． Key words： sputum；acid—fast bacilli；centrifuging the liquored sputa and collecting bacteria；Pulmonary tuberculosis 痰抗酸杆菌检查阳性是诊断肺结核的重要依据。痰涂片镜检对发现结核病传染源、选择治疗方案、考核疗效、评价防治效果具有非常重要的作用。如何提高痰抗酸杆菌检查的阳性率，是结核病防治工作中亟待解决的关键问题之一。作者对新乡医学院第一附属医院结核科住院患者的痰标本，同时采用夹层杯集菌离心涂片法及普通涂片法检测抗酸杆菌，对比二者的痰菌阳性率，以期获得新的痰菌检测方法，现报道如下。 1 资料与方法 1．1 一般资料 取新乡医学院第一附属医院结核科2006年8月至2007年2月的住院患者614例，其中男392例(63．8％)，女222例(36．2％)，年龄18—70岁，平均(41±1．8)岁。所有人选病例均符合《中华人民共和国传染病防治法》规定管理的传染病诊断标准(试行)，并且经过临床观察3个月证实诊断为肺结核无误者 1-2]。614例患者的痰液先后均采用夹层杯集菌离心涂片法及普通涂片法检测抗酸杆菌。 1．2 试剂和仪器 夹层杯法抗酸染色液所用彭氏 杯为湘药管械(准)字2005第2410002；其专