具共光程移相干涉术之表面电浆共振显微仪(Acommon-pathphase.doc

具共光程移相干涉術之表面電漿共振顯微儀(A common-path phase-shift interferometry surface plasmon resonance microscope) 蘇園登，陳顯禎* 國立成功大學工程科學系 台南市701大學路一號 Y.-T. Su and S.-J. Chen* Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan. *Tel: +886-6-2757575 ext. 63351; Fax: +886-6-2766549; E-mail: sheanjen@mail.ncku.edu.tw. 摘要 (surface plasmon resonance，SPR)y，PSI)e，SPW)形成共振現象，而且SPR現象會隨界面條件微量改變而造成反射相位驟變，同時利用S偏振光相位保持不變為參考光，將兩者形成干涉圖案，利用電光調變器(electro-optical modulation)來造成相位移干涉，並經由相位重建方法取得空間相位之變化情形。此共光程相位技術可減低外界環境擾動、機械振動與雷射不穩定等因素，提供長時間穩定之相位量測(於兩個小時內可達10-3π之穩定度)，目前此系統可精密偵測到1x10-6π RIU折射率之變化，其空間側向解析度(lateral resolution)約10μm左右。 Abstract A common-path surface plasmon resonance (SPR) phase-shift interferometry (PSI) imaging system is proposed based on the integration of SPR and common-path PSI techniques to measure the 2-D spatial phase variation caused by biomolecular interactions upon a sensing chip. The common-path SPR-PSI imaging system offers high resolution, high-throughout screening, and long-term stability capabilities to analyze microarray biomolecular interaction without the need for additional labeling, and provides valuable real-time quantitative information. Currently, the common-path PSI technique by means of a five-step phase reconstruction method with a liquid crystal device (LCD) can provide an extreme long-term stability (這段有點像廣告文字，若投稿的目標非常technical, 要不要換成中文版 說明為什麼 會有這樣好的特性, 的那一段) even with external disturbances such as mechanical vibration, buffer flow noise, and laser unstable issue to match the demand of real-time kinetic study in drug discovery. The SPR-PSI imaging system has achieved a detection limit of 1 × 10-6 refraction index change, a long-term phase stability of 10-3 ( in over 120 minutes, and a spatial phase resolution of 2 x 10-3 ( with a lateral resolution of 50μm. 一、前言 1. INTRODUCTION 表面電漿共振(surface plasmon resonance，SPR)感測器可量測在固體與液體或固體與氣體界面之奈米膜層之介電常數或厚度的微量變化[1,2]。由於SPR技術具有快速地、靈敏度高、動態分析、無須對待測分子做任何的標記及定性和定量生物分子交互作用之優點，自1992年來便被廣泛地應用