梅州地区小儿感染肺炎支原体生物群分布与耐药性差异研究.pdfVIP

898 中国人兽共患病学报 Chinese J ournal of Zoonoses 001: 10. 3969/j. issn. 1002- 2694.2016.010.010 梅州地区小儿感染肺炎支原体生物群 分布与耐药性差异研究 肖光文l ，李舟文l ，张国雄2 ，徐美兰 1 ，乔亚峰1 ，曾令斌1 2016 , 32 (1 0) 摘 要 g 目的 了解梅州地区儿童肺炎支原体流行株的基因分型及不同型别间对大环内醋类药物的耐药性差异。方法 对 2015 年 1 月到 2015 年 12 月期间 865 例小儿肺炎患儿进行咽拭子肺炎支原体培养，并检测对红霉素、罗红霉素、阿奇霉索、 乙耽螺旋霉素、克林霉素和克拉霉素 6 种药物的敏感性。采用基于 MpP1 基因的巢式多重 PCR 方法进行 Mρ 基因亚型的监 测并分析不同基因型对大环内醋类药物的耐药性差异。结果 对肺炎支原体培养的结果表明 ，Mp 培养阳性数为 289 例，总 阳性率为 33.41 %。其中 P1-1 型为 246 例，占 85.12% ， Pl-II 型为 43 例，占 14.88% ， 196 例出现大环内醋类抗菌药物耐药的 Mp 阳性标本中，P1-1 型为 191 例，占 P1-1 型的 77.64% ;P1-II 型仅 5 例，占 P1-II 型的 1 1. 63% ， P1-1 型的耐药率明显高于 Pl-II 型 (χ2 =73. 09 ,P0. 0 1) 。结论 Mp 仍是梅州地区儿童肺炎的主要病原体之一，其流行基因型以 Pl-I型为主，同时也 存在 P1-II 型菌株;在控制 Mρ 的感染方面应重视耐药菌株的克隆传播。 关键词:肺炎支原体;儿童;基因型;耐药性差异 申图分类号 :R375 文献标i只码 :A 文章编号: 1002 - 2694 (20 16) 10-0898-05 Mycoplasma pneumoniae serotypes distribution and drug resistance in Meizhou area , China XIAO Guang-wen 1 , LI Zhou-wen 1 , ZHANG Guo-xiong 2 , XU Mei-lan 1 , QIAO Ya-feng 1 , ZENG Ling-bin 1 (1. Medical College , ] iaying University , Meizhou 514031 , China; 2. Clinical Laboratory , the People 气 Hosρital ， Meizhou 514000 , China) Abstract: In order to survey the genotype of Mycoplasma ρneumonia (Mp) strains isolated from children with Mp infec- tion and the drug resistance difference of genotypes to large ring lactone class of drugs in Meizhou area , a total of 865 throat swabs were collected from children with pneumonia in Meizhou area from January 2015 to December 2015. The swabs were cul- tured to detect Mρ. The drug sensitivity of M户 to erythromycin , roxithromycin , azithromycin , acetylspiramycin , clindamycin and clarithromycin was evaluated. The nested-multiplex PCR based on MP P1 gene was performed to detect the subtype of MP gene , and the drug resistance difference of genotypes was researched to large ring lactone class of drugs. According to the re- sult of the cultivation of M户， 289 (33.41%) were Mp-positive , the infection rate of spring and summer was sign