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干眼症疾病相关的细胞因子研究
Correlation of Tear Inflammatory Cytokines and Matrix
Metalloproteinases with Four Dry Eye Diagnostic Tests
Karl R. VanDerMeid,1 Stephanie P. Su,2 Keith W. Ward,1 and Jin-Zhong Zhang1
PURPOSE. Tear cytokines and matrix metalloproteinases (MMPs)
can be extracted from the Schirmer strip. This study examined
the extracted levels of tear cytokines and MMPs from Schirmer
strips and potential correlation with Schirmer’s test, tear
breakup time (TBUT), tear osmolarity, and ocular surface dis-
ease index (OSDI).
METHODS. Thirty healthy volunteers were clinically evaluated
for known methods to diagnose dry eye disease, including
Schirmer’s test, tear osmolarity, OSDI, and TBUT. Tears were
collected by Schirmer strips and proteins were extracted from
the Schirmer strip in 0.5 M NaCl with 0.5% Tween 20 and
analyzed using multiplex assay kits to examine cytokines or
MMPs. Calculated cytokine and MMP concentrations for all
samples were sorted into groups according to a positive or
negative for each of the above-cited four dry eye diagnostic
tests, individually and in combination.
RESULTS. Five inflammatory cytokines (IL-1, -1, -6, -8, and
TNF-) and five MMPs (MMPs 1, 2, 7, 9, and 10) were extracted
from clinical Schirmer strips. Schirmer strip measurement and
tear osmolarity correlated well with increased concentrations
of the inflammatory cytokines and MMPs, whereas TBUT and
OSDI did not.
CONCLUSIONS. Both the Schirmer’s test and tear osmolarity may
be more relevant to the clinician in the diagnosis of ocular
surface diseases with an increased level of inflammatory
mediators. (Invest Ophthalmol Vis Sci. 2012;53:1512–1518)
DOI:10.1167/iovs.11-7627
Tear samples are being used with increasing frequency todetect biomarkers of normal and diseased states of the
ocular surface,1–3 including allergy4 and dry eye.5,6 Although
use of ELISA is common, the use of multiplex bead,7,8 multi-
array, and proteomic9–11 technology has enhanced the use of
collected tears by allowing for analysi
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