一株产红色素真菌的初步鉴定及色素提取与稳定性研究 毕业设计.docVIP

学科分类号 本 科 毕 业 论 文 题 目 一株姓 名 学 号 院 (系) 化学与生命科学学院 专 业 生物资源科学 年 级 2011级 指导教师 职 称 副教授 二 二一五 年 五 月 二 贵州师范学院本科毕业论文诚信声明 本人郑重声明：所呈交的本科毕业论文，是本人在指导老师的指导下，独立进行研究工作所取得的成果，成果不存在知识产权争议，除文中已经注明引用的内容外，本论文不含任何其他个人或集体已经发表或撰写过的作品成果。对本文的研究做出重要贡献的个人和集体均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。 本科毕业论文作者签名： 年月日 目录 摘 要 真菌色素是一种极具应用潜力的食用色素。本文针对一株产红色素的真菌，对菌株进行了鉴定、色素提取工艺和色素稳定性方面的研究。实验结果表明：该菌株通过形态学鉴定为酒红拟青霉Paecilomyces vinaceus)。菌株所产红色素，其最大吸收波长为510 nm色素提取时，以鲜菌丝直接浸提，最佳提取剂为丙酮，提取时间为240 min，提取温度为30 ℃；提取时对菌丝进行超声波破碎预处理有助于色素的提取。该色素在中性和弱酸弱碱条件下的稳定性较好；温度糖类对色素稳定性影响；金属离子Mg2+、Cu2+、Na+、K+、Fe3+、Zn2+Fe2+、Ca2+对色素 Abstract Fungal pigments were potential resource for natural food colorant. In this article, an unknown fungus stain A11625M, which can produce red pigment, was studied on its identification, pigment extraction and the pigment stability. The results showed that the strain was identified as Paecilomyces vinaceus by morphological character. The maximum absorption wavelengh of the pigment, which produced by the strain, was 510 nm. Fresh mycelium was more suitable for pigment extraction than dry mycelium, the best extraction solvent was acetone, and the optimal extraction time was 240 minutes, the optimal extraction temperature was 30 ℃. In addition, crushing the mycelium could be helpful for pigment extraction. The pigment was stable in neutral, weakly acidic and alkalescent condition. Temperature had little effect on the stability of the pigment. Carbohydrate had no influence on the pigment. Metallic ions, such as Mg2+, Cu2+, Na+, K+, Fe3+, Zn2+, Fe2+, Ca2+ had little influence on the pigment; H2O2 influenced the pigment significantly; The Na2SO3 had little influence on the p