2007_Isolation and characterization of antibacterial substances.pdf

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2007_Isolation and characterization of antibacterial substances.pdf

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2007_Isolation and characterization of antibacterial substances

Isolation and characterization of antibacterial substances produced by marine actinomycetes in the presence of seawater Chiaki Imada1, Naoko Koseki1, Masazumi Kamata2, Takeshi Kobayashi1, and Naoko Hamada-Sato1 1Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan 2Department of Science of Aesthetics and Art, Yamano College of Aesthetics, 530, Yarimizu, Hachioji, Tokyo 192-0396, Japan (Received Jul. 31, 2006 / Accepted Apr. 4, 2007 / Published Jun. 14, 2007) Actinomycetes, which produced antibacterial substances against Gram-positive bacteria in the presence of seawater only, were isolated from marine environment and characterized. Of the 100 isolates from Otsuchi Bay in Iwate, 41 failed to produce any antibacterial substances in the absence of seawater. However, of the 41, two were found to produce antibacterial substances in the presence of seawater. Strain No. 18 exhibited higher activity than strain No. 28 and was, therefore, selected for further studies. Phylogenetic analysis and physiological characterization showed a high similarity between strain No. 18 and Micromonospora globosa. However, M. globosa JCM 3126 did not produce any antibiotics in the presence of seawater. Therefore, the effect of seawater on the growth of strain No. 18 and its production of antibacterial substances was inves- tigated. Strain No. 18 grew in a medium prepared with artificial seawater at a concentration range of 0– 140% (v/v). The optimal concentration of growth ranged from 10 to 30% (v/v). However, production of antibacterial substances was observed in the concentration range 60–110% (v/v) despite poor growth. Thus, the production of antibacterial substances is seawater-dependent. The purification and characterization of the substances is now in progress. INTRODUCTION Natural organic compounds produced by microorgan- isms are an important screening target for a variety of bioactive sub