Aqueous two-phase extraction for protein recovery from corn extracts.pdf

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Aqueous two-phase extraction for protein recovery from corn extracts.pdf

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Aqueous two-phase extraction for protein recovery from corn extracts

Ai p p m f l s t s p t a A r ? K 1 e i p t n t n w s c 1 dJournal of Chromatography B, 845 (2007) 38–50 Aqueous two-phase extraction for protein recovery from corn extracts Zhengrong Gu, Charles E. Glatz ? Department of Chemical and Biological Engineering, 2114 Sweeney Hall, Iowa State University, Ames, IA 50011-2230, USA Received 27 April 2006; accepted 17 July 2006 Available online 22 August 2006 bstract Corn has been used as an expression host for several recombinant proteins with potential for large-scale production. Cost-effective downstream nitial recovery, separation and concentration remain a challenge. Aqueous two-phase (ATP) partitioning has been used to recover and concentrate roteins from fermentation broths and offers advantages for integration of those steps with biomass removal. To examine the applicability of ATP artitioning to recombinant protein purification from corn endosperm and germ, ATP system parameters including poly(ethylene glycol) (PEG) olecular weight (MW), phase-forming salt, tie line length (TLL), and pH were manipulated to control partitioning of extracted native proteins rom each fraction. Moderate PEG MW, reduction of phase ratio, and added NaCl effected complete recovery of the hydrophobic model protein ysozyme in the top phase with ca. 5× enrichment and illustrates a favorable match of recombinant protein characteristics, expression host, and eparation method. Furthermore, integration of protein extraction with the partitioning reduced the load of contaminating host proteins relative to he more traditional separate steps of extraction followed by partitioning. Performance of the integrated partitioning was hindered by endosperm olids loading, whereas for germ, which has ca. 35× higher aqueous soluble protein, the limit was protein solubility. For more hydrophilic model roteins (the model being cytochrome c), effective separation required further reduction of PEG MW to effect more partitioning of host proteins to he top phase and e