Folic acid-conjugated iron oxide porous nanorods yuping.pdf

F d P L K a A R R A A K P D A A 1 s m h e h s t a O a d f n s a h 0Colloids and Surfaces B: Biointerfaces 120 (2014) 142–151 Contents lists available at ScienceDirect Colloids and Surfaces B: Biointerfaces jo ur nal ho me p ag e: www.elsev ier .com/ locate /co lsur fb olic acid-conjugated iron oxide porous nanorods loaded with oxorubicin for targeted drug delivery ing Yu, Xi-Ming Xia, Ming Wu, Can Cui, Yang Zhang, Lei Liu, Bo Wu, Cai-Xia Wang, iu-Jie Zhang, Xiang Zhou, Ren-Xi Zhuo, Shi-Wen Huang ? ey Laboratory of Biomedical Polymers, Ministry of Education; Department of Chemistry, Wuhan University, Wuhan 430072, Hubei, PR China r t i c l e i n f o rticle history: eceived 24 February 2014 eceived in revised form 4 May 2014 ccepted 9 May 2014 vailable online 22 May 2014 eywords: orous nanorods rug delivery nti-cancer a b s t r a c t Iron oxide porous nanorods (IOPNR) with lengths ranging from 40 nm to 60 nm and pore diameters ran- ging from 5 nm to 10 nm were prepared, and further modified with NH2–PEG–FA (FA–PEG–IOPNR) for ligand targeting and modified with NH2–PEG–OCH3 (PEG–IOPNR) as a control. Instead of chemical bond- ing, doxorubicin (DOX), a low water solubility anticancer drug, was loaded in the pores of the modified IOPNR because of their porous structure and high porosity. The release of DOX in acidic PBS solution (pH 5.3) was faster than that in neutral (pH 7.4) solution. The analysis results from TEM, inductively coupled plasma emission spectroscopy, confocal laser scanning microscopy, and flow cytometry analyses indi- cated that the presence of FA on the surface of the nanorods increase the cellular uptake of nanorods in the case of HeLa cells, a folate receptor (FR)-positive cell line. In contrast, for COS 7 cells, a FR-negativective targeting cell line, FA ligand on the su