Fractionation and purification of the polysaccharides with marked antitumor activity.pdf

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Fractionation and purification of the polysaccharides with marked antitumor activity.pdf

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Fractionation and purification of the polysaccharides with marked antitumor activity

1970;30:2776-2781. Published online November 1, 1970.Cancer Res Goro Chihara, Junji Hamuro, Yukiko Y. Maeda, et al. (Berk.) Sing. (an Edible Mushroom)edodes LentinusMarked Antitumor Activity, Especially Lentinan, from Fractionation and Purification of the Polysaccharides with Updated Version /content/30/11/2776 Access the most recent version of this article at: Citing Articles /content/30/11/2776#related-urls This article has been cited by 4 HighWire-hosted articles. Access the articles at: E-mail alerts related to this article or journal.Sign up to receive free email-alerts Subscriptions Reprints and .pubs@Department at To order reprints of this article or to subscribe to the journal, contact the AACR Publications Permissions .permissions@Department at To request permission to re-use all or part of this article, contact the AACR Publications American Association for Cancer Research Copyright ? 1970 on July 13, 2011Downloaded from [CANCER RESEARCH 30, 2776-2781, November 1970] Fractionation and Purification of the Polysaccharides with Marked Antitumor Activity, Especially Lentinan, from Lentinus edodes (Berk.) Sing, (an Edible Mushroom)1 Goro Chihara, Junji Hamuro, Yukiko Y. Maeda, Yoshiko Arai, and Fumiko Fukuoka National Cancer Center Research Institute, Tsukiji, Tokyo, Japan SUMMARY Six polysaccharide preparations, lentinan, LC-11, LC-12, LC-13, EC-11, and EC-14, were isolated from the water- soluble extracts of Lentinus edodes (Berk.) Sing., an edible mushroom, by fractional precipitation with ethanol and cetyl- trimethylammonium hydroxide, fractional solubilization with acetic acid, and diethylaminoethyl cellulose column chroma- tography. The first two polysaccharide preparations have a strong anti- tumor effect; lentinan, especially, markedly inhibited the growth of Sarcoma 180 implanted s.c. in mice, inducing almost complete regression of tumors at doses of 1 mg/kg X 10 with no sign of toxicity. LC-11 was also effective in 5