IJP-Insulin gel.pdfVIP

PN o Y a b a A R R A A K L T I M D 1 i p i t c t p i fi s d S w p o 2 w d m 0 hInternational Journal of Pharmaceutics 437 (2012) 51– 56 Contents lists available at SciVerse ScienceDirect International Journal of Pharmaceutics jo ur nal homep a ge: www.elsev ier .com/ locate / i jpharm harmaceutical Nanotechnology ovel lyophilized hydrogel patches for convenient and effective administration f microneedle-mediated insulin delivery uqin Qiua, Guangjiong Qina,b, Suohui Zhanga, Yan Wua,b, Bai Xua, Yunhua Gaoa,? Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China Graduate University Chinese Academy of Sciences, Beijing 100049, China r t i c l e i n f o rticle history: eceived 2 February 2012 eceived in revised form 29 June 2012 ccepted 22 July 2012 vailable online xxx a b s t r a c t A lyophilized hydrogel patch system was developed for microneedle-mediated insulin delivery. The matrix of Cross-linked poly(acrylamide-co-acrylic acid) were synthesized by precipitation polymer- ization. Recombinant human Insulin was loaded into the lyophilized polymer matrix, which can be rehydrated by water. After the hydrated patch was applied to the abdominal skin of diabetic rats after microneedle pretreatment, pharmacodynamics and pharmacokinetics evaluation was performed. Theeywords: yophilized hydrogel ransdermal drug delivery nsulin icroneedle iabetes blood samples were collected to monitor blood glucose and serum insulin levels for 12 h. Blood glu- cose was lowered in proportion to the concentration of insulin loaded in lyophilized hydrogel patches (R2 = 0.99), with a longer duration of action compared to subcutaneous injection. Stability study con- firmed more than 90% of insulin activity was retained in lyophilized hydrogel