ExonShuffling-1 外显子重组.pdf

1Origins of New Genes: Exon Shuffling By Carl Hillstrom 2? The talk is about how the shuffling of exons can give rise to new genes. 3Merriam-Webster Online Dictionary Main Entry: ex·on Pronunciation: ek-s?n Function: noun : a polynucleotide sequence in a nucleic acid that codes information for protein synthesis and that is copied and spliced together with other such sequences to form messenger RNA -- compare INTRON /dictionary/exon 4Exon shuffling Recombination, exclusion, or duplications of exons can drive the evolution of new genes. The general idea of exon shuffling is typically attributed to Walter Gilbert (e.g. Long et al. 2003) The definition of exon shuffling used in this presentation encompass: --exon assumes a new function after it has been moved --exon retains its original function after it has been moved 5? So what is exon shuffling? ? It is basically the idea that recombination or exclusion of exons can drive the evolution of new genes. “Recombination, exclusion, or duplications of exons can drive the evolution of new genes.” –this is a very general definition that I have adopted for the purpose of this presentation. ? The definition of exon shuffling used in this presentation encompass: ? --exon assumes a new function after it has been moved ? --exon retains its original function after it has been moved ? there is disagreement whether exon shuffling applies to both of these definition—for simplicity, I will use the concept of exon shuffling as if it applies to both of these definitions 6Outline of a typical antibody Alberts et al. (2002) A concrete example of how exon shuffling is physiologically crucial. The immunoglobulin genes of undifferentiated carries broad coding capacity. But through deletions and rearrangements of the gene as B lymphocytes differentiate, considerable functional diversity can be conferred. /books/bv.fcgi?rid=mboc4.figgrp.1421 7A concrete example of how exon shuffling is physiologically crucial. The immun